Microtubule stabilization by bone morphogenetic protein receptor-mediated scaffolding of c-Jun n-Terminal kinase promotes dendrite formation

Monika Podkowa, Xin Zhao, Chi Wing Chow, Eleanor T. Coffey, Roger J. Davis, Liliana Attisano

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Neuronal outgrowth occurs via coordinated remodeling of the cytoskeleton involving both actin and microtubules. Microtubule stabilization drives the extending neurite, yet little is known of the molecular mechanisms whereby extracellular cues regulate microtubule dynamics. Bone morphogenetic proteins (BMPs) play an important role in neuronal differentiation and morphogenesis, and BMP7 in particular induces the formation of dendrites. Here, we show that BMP7 induces stabilization of microtubules in both a MAP2-dependent neuronal cell culture model and in dendrites of primary cortical neurons. BMP7 rapidly activates c-Jun N-terminal kinases (JNKs), known regulators of microtubule dynamics, and we show that JNKs associate with the carboxy terminus of the BMP receptor, BMPRII. Activation and binding of JNKs to BMPRII is required for BMP7-induced microtubule stabilization and for BMP7-mediated dendrite formation in primary cortical neurons. These data indicate that BMPRII acts as a scaffold to localize and coordinate cytoskeletal remodeling and thereby provides an efficient means for extracellular cues, such as BMPs, to control neuronal dendritogenesis.

Original languageEnglish (US)
Pages (from-to)2241-2250
Number of pages10
JournalMolecular and Cellular Biology
Volume30
Issue number9
DOIs
StatePublished - May 2010

Fingerprint

Bone Morphogenetic Protein Receptors
Dendrites
Microtubules
Phosphotransferases
JNK Mitogen-Activated Protein Kinases
Bone Morphogenetic Proteins
Cues
Neurons
Neurites
Cytoskeleton
Morphogenesis
Actins
Cell Culture Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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Microtubule stabilization by bone morphogenetic protein receptor-mediated scaffolding of c-Jun n-Terminal kinase promotes dendrite formation. / Podkowa, Monika; Zhao, Xin; Chow, Chi Wing; Coffey, Eleanor T.; Davis, Roger J.; Attisano, Liliana.

In: Molecular and Cellular Biology, Vol. 30, No. 9, 05.2010, p. 2241-2250.

Research output: Contribution to journalArticle

Podkowa, Monika ; Zhao, Xin ; Chow, Chi Wing ; Coffey, Eleanor T. ; Davis, Roger J. ; Attisano, Liliana. / Microtubule stabilization by bone morphogenetic protein receptor-mediated scaffolding of c-Jun n-Terminal kinase promotes dendrite formation. In: Molecular and Cellular Biology. 2010 ; Vol. 30, No. 9. pp. 2241-2250.
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