Microtubule organization and function in epithelial cells

Research output: Contribution to journalReview article

177 Scopus citations

Abstract

Microtubules are essential for many aspects of polarity in multicellular organisms, ranging from the asymmetric distribution of cell-rate determinants in the one-cell embryo to the transient polarity generated in migrating fibroblasts. Epithelial cells exhibit permanent cell polarity characterized by apical and basolateral surface domains of distinct protein and lipid composition that are segregated by tight junctions. They are also endowed with a microtubule network that reflects the asymmetry of their cell surface: microtubule minus-ends face the apical- and microtubule plus-ends the basal domain. Strikingly, the formation of distinct surface domains during epithelial differentiation is accompanied by the re-organization of microtubules from a uniform array focused at the centrosome to the noncentrosomal network that aligns along the apico-basolateral polarity axis. The significance of this coincidence for epithelial morphogenesis and the signaling mechanisms that drive microtubule repolymerization in developing epithelia remain major unresolved questions that we are only beginning to address. Studies in cultured polarized epithelial cells have established that microtubules serve as tracks that facilitate targeted vesicular transport. Novel findings suggest, moreover, that microtubule-based transport promotes protein sorting, and even the generation of transport carriers in the endo- and exocytic pathways.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalTraffic
Volume5
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Keywords

  • Apical surface
  • Epithelial polarity
  • Microtubules
  • Polarized protein transport

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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