Microsatellite analysis and telomerase activity in archived tissue and urine samples of bladder cancer patients

Jürgen F. Linn, Miriam Lango, Sarel Halachmi, Mark P. Schoenberg, David Sidransky

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We performed microsatellite analysis and tested telomerase activity in paired tissue and urine of bladder cancer patients from frozen archived samples. DNA obtained from microdissected tumor and urine sediment was analyzed and compared to peripheral lymphocytes for microsatellite alterations (loss of heterozygosity [LOH] or instability) using a panel of 20 microsatellite markers in 15 patients with transitional or squamous cell carcinoma of the urinary tract. Additionally, telomerase activity was determined in 12 microdissected tumor specimens and corresponding frozen urine pellets. Tumor cell DNA was detected by microsatellite analysis (LOH or shift) in at least one marker in 14/15 microdissected tumor specimens and in 13/15 DNA samples obtained from urine sediments. Telomerase activity was present in 11/12 tumor samples but could not be detected in any of the corresponding urine sediments. Frozen archived urine samples are useful for retrospective studies utilizing microsatellite analysis or other PCR-based approaches after DNA extraction. However, the evaluation of telomerase protein activity in stored urine samples appears to be unsuitable.

Original languageEnglish (US)
Pages (from-to)625-629
Number of pages5
JournalInternational Journal of Cancer
Volume74
Issue number6
DOIs
StatePublished - Dec 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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