MicroRNAs are expressed and processed by human primary macrophages

Aimée J. Luers, Olivier D. Loudig, Joan W. Berman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Macrophages are crucial to host defense, functioning in innate and cell-mediated immunity. MicroRNAs (miRNAs) are small non-coding RNA molecules that repress transcription and protein production. Little is known about miRNA expression in primary human macrophages, or about how macrophage miRNAs contribute to both normal macrophage function and to the pathogenesis of disease in humans. Using Western blot analyses, we demonstrated the production of miRNA machinery proteins by human primary macrophages. Using two different miRNA array techniques, we identified 119 miRNAs expressed by human primary macrophages, including hsa-let-7a, miR-16, -23a, 30b, -103, -146a, -212, and -378 and validated them by quantitative RT-PCR. Our findings provide a knowledge base to which macrophage miRNA expression in organ-specific macrophages or disease processes may be compared in humans.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalCellular Immunology
Volume263
Issue number1
DOIs
StatePublished - 2010

Fingerprint

MicroRNAs
Macrophages
Small Untranslated RNA
Knowledge Bases
Cellular Immunity
Proteins
Western Blotting
Polymerase Chain Reaction

Keywords

  • Argonaute2
  • Dicer
  • Drosha
  • MiRNA machinery
  • MiRNA variability
  • MiRNome
  • Monocyte derived macrophage

ASJC Scopus subject areas

  • Immunology

Cite this

MicroRNAs are expressed and processed by human primary macrophages. / Luers, Aimée J.; Loudig, Olivier D.; Berman, Joan W.

In: Cellular Immunology, Vol. 263, No. 1, 2010, p. 1-8.

Research output: Contribution to journalArticle

Luers, Aimée J. ; Loudig, Olivier D. ; Berman, Joan W. / MicroRNAs are expressed and processed by human primary macrophages. In: Cellular Immunology. 2010 ; Vol. 263, No. 1. pp. 1-8.
@article{4661655823794ce58057605046a8ffd0,
title = "MicroRNAs are expressed and processed by human primary macrophages",
abstract = "Macrophages are crucial to host defense, functioning in innate and cell-mediated immunity. MicroRNAs (miRNAs) are small non-coding RNA molecules that repress transcription and protein production. Little is known about miRNA expression in primary human macrophages, or about how macrophage miRNAs contribute to both normal macrophage function and to the pathogenesis of disease in humans. Using Western blot analyses, we demonstrated the production of miRNA machinery proteins by human primary macrophages. Using two different miRNA array techniques, we identified 119 miRNAs expressed by human primary macrophages, including hsa-let-7a, miR-16, -23a, 30b, -103, -146a, -212, and -378 and validated them by quantitative RT-PCR. Our findings provide a knowledge base to which macrophage miRNA expression in organ-specific macrophages or disease processes may be compared in humans.",
keywords = "Argonaute2, Dicer, Drosha, MiRNA machinery, MiRNA variability, MiRNome, Monocyte derived macrophage",
author = "Luers, {Aim{\'e}e J.} and Loudig, {Olivier D.} and Berman, {Joan W.}",
year = "2010",
doi = "10.1016/j.cellimm.2010.03.011",
language = "English (US)",
volume = "263",
pages = "1--8",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - MicroRNAs are expressed and processed by human primary macrophages

AU - Luers, Aimée J.

AU - Loudig, Olivier D.

AU - Berman, Joan W.

PY - 2010

Y1 - 2010

N2 - Macrophages are crucial to host defense, functioning in innate and cell-mediated immunity. MicroRNAs (miRNAs) are small non-coding RNA molecules that repress transcription and protein production. Little is known about miRNA expression in primary human macrophages, or about how macrophage miRNAs contribute to both normal macrophage function and to the pathogenesis of disease in humans. Using Western blot analyses, we demonstrated the production of miRNA machinery proteins by human primary macrophages. Using two different miRNA array techniques, we identified 119 miRNAs expressed by human primary macrophages, including hsa-let-7a, miR-16, -23a, 30b, -103, -146a, -212, and -378 and validated them by quantitative RT-PCR. Our findings provide a knowledge base to which macrophage miRNA expression in organ-specific macrophages or disease processes may be compared in humans.

AB - Macrophages are crucial to host defense, functioning in innate and cell-mediated immunity. MicroRNAs (miRNAs) are small non-coding RNA molecules that repress transcription and protein production. Little is known about miRNA expression in primary human macrophages, or about how macrophage miRNAs contribute to both normal macrophage function and to the pathogenesis of disease in humans. Using Western blot analyses, we demonstrated the production of miRNA machinery proteins by human primary macrophages. Using two different miRNA array techniques, we identified 119 miRNAs expressed by human primary macrophages, including hsa-let-7a, miR-16, -23a, 30b, -103, -146a, -212, and -378 and validated them by quantitative RT-PCR. Our findings provide a knowledge base to which macrophage miRNA expression in organ-specific macrophages or disease processes may be compared in humans.

KW - Argonaute2

KW - Dicer

KW - Drosha

KW - MiRNA machinery

KW - MiRNA variability

KW - MiRNome

KW - Monocyte derived macrophage

UR - http://www.scopus.com/inward/record.url?scp=77952546939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952546939&partnerID=8YFLogxK

U2 - 10.1016/j.cellimm.2010.03.011

DO - 10.1016/j.cellimm.2010.03.011

M3 - Article

C2 - 20403586

AN - SCOPUS:77952546939

VL - 263

SP - 1

EP - 8

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -