Methylmercury has a selective effect on mitochondria in cultured astrocytes in the presence of [U-13C]glutamate

Jeffrey W. Allen, Haytham El-Oqayli, Michael Aschner, Tore Syversen, Ursula Sonnewald

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The effect of methylmercury on glutamate metabolism was studied by 13C magnetic resonance spectroscopy. Cerebral cortical astrocytes were pretreated with methylmercury, either 1 μM for 24 h, or 10 μM for 30 min, and subsequently with 0.5 mM [U-13C]glutamate for 2 h. Labeled glutamate, glutamine, aspartate and glutathione were present in cell extracts, and glutamine, aspartate and lactate in the medium of all groups. HPLC analysis of these amino acids showed no changes in concentrations between groups. Surprisingly, the amounts of [U-13C]glutamate and unlabeled glucose taken up by the astrocytes were unchanged. Furthermore, the amounts of most metabolites synthesized from [U-13C]glutamate were also unchanged in all groups. However, formation of [U-13C]lactate was decreased in the 10 μM methylmercury group. This was not observed for labeled aspartate. It is noteworthy that both [U-13C]lactate and [U-13C]aspartate can only be derived from [U-13C]glutamate via mitochondrial metabolism. [U-13C]glutamate enters the tricarboxylic acid cycle (located in mitochondria) after conversion to 2-[U-13C]oxoglutarate and [U-13C]aspartate is formed from [U-13C]oxaloacetate, as is [U-13C]lactate. [U-13C]lactate can also be formed from [U-13C]malate. This differential effect on labeled aspartate and lactate indicates cellular compartmentation and thus selective vulnerability of mitochondria within the astrocytes to the effects of methylmercury. The decreased lactate production from glutamate might be detrimental to surrounding cells since lactate has been shown to be an important substrate for neurons.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalBrain Research
Volume908
Issue number2
DOIs
StatePublished - Jul 27 2001
Externally publishedYes

Fingerprint

Astrocytes
Glutamic Acid
Lactic Acid
Mitochondria
Aspartic Acid
Glutamine
Ketoglutaric Acids
Oxaloacetic Acid
Citric Acid Cycle
Cell Extracts
Glutathione
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
Neurons
Amino Acids
Glucose

Keywords

  • Astrocytes
  • Glutamate
  • Methylmercury
  • Mitochondria
  • MR spectroscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Methylmercury has a selective effect on mitochondria in cultured astrocytes in the presence of [U-13C]glutamate. / Allen, Jeffrey W.; El-Oqayli, Haytham; Aschner, Michael; Syversen, Tore; Sonnewald, Ursula.

In: Brain Research, Vol. 908, No. 2, 27.07.2001, p. 149-154.

Research output: Contribution to journalArticle

Allen, Jeffrey W. ; El-Oqayli, Haytham ; Aschner, Michael ; Syversen, Tore ; Sonnewald, Ursula. / Methylmercury has a selective effect on mitochondria in cultured astrocytes in the presence of [U-13C]glutamate. In: Brain Research. 2001 ; Vol. 908, No. 2. pp. 149-154.
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