TY - JOUR
T1 - Methylation in embryonic stem cells in vitro.
AU - Nishino, Koichiro
AU - Ohgane, Jun
AU - Suzuki, Masako
AU - Hattori, Naka
AU - Shiota, Kunio
PY - 2006
Y1 - 2006
N2 - Stem cells raise the possibility of regenerating failing body parts with new tissue. Before stem cells can safely fulfill their promise, many technical problems, including understanding the stem cell phenotype, must be overcome. DNA methylation, which is responsible for gene silencing and is associated with chromatin remodeling, is an epigenetic system that determines the specific characteristic of a variety of cells, including stem cells. Each cell type has a unique DNA methylation profile produced by varied loci-specific methylation. Investigation of such DNA methylation profiles provides a way of identifying pluripotent stem cells. Further, it is likely that analysis of the epigenetic status of stem cells may provide novel information regarding "sternness" within these populations.
AB - Stem cells raise the possibility of regenerating failing body parts with new tissue. Before stem cells can safely fulfill their promise, many technical problems, including understanding the stem cell phenotype, must be overcome. DNA methylation, which is responsible for gene silencing and is associated with chromatin remodeling, is an epigenetic system that determines the specific characteristic of a variety of cells, including stem cells. Each cell type has a unique DNA methylation profile produced by varied loci-specific methylation. Investigation of such DNA methylation profiles provides a way of identifying pluripotent stem cells. Further, it is likely that analysis of the epigenetic status of stem cells may provide novel information regarding "sternness" within these populations.
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M3 - Article
C2 - 16846008
AN - SCOPUS:33747591942
SN - 1064-3745
VL - 329
SP - 421
EP - 445
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -