Methods to study monocyte migration induced by HIV-infected cells

Vasudev R. Rao, Eliseo A. Eugenin, Joan W. Berman, Vinayaka R. Prasad

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.

Original languageEnglish (US)
Title of host publicationHIV Protocols
EditorsVinayaka R. Prasad, Ganjam V. Kalpana
Pages295-309
Number of pages15
DOIs
StatePublished - Feb 23 2009

Publication series

NameMethods in Molecular Biology
Volume485
ISSN (Print)1064-3745

Keywords

  • HIV-associated dementia/monocyte migration/monocyte chemotaxis/HIV-1 Tat/monocyte chemotaxis protein-1/chemokines

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Rao, V. R., Eugenin, E. A., Berman, J. W., & Prasad, V. R. (2009). Methods to study monocyte migration induced by HIV-infected cells. In V. R. Prasad, & G. V. Kalpana (Eds.), HIV Protocols (pp. 295-309). (Methods in Molecular Biology; Vol. 485). https://doi.org/10.1007/978-1-59745-170-3_20