Methods for monitoring autophagy from yeast to human

Daniel J. Klionsky, Ana Maria Cuervo, Per O. Seglen

492 Scopus citations

Abstract

The increasing interest in autophagy in a wide range of organisms, accompanied by an ever-growing influx of researchers into this field, necessitates a good understanding of the methodologies available to monitor this process. In this review we discuss current approaches that can be used to follow the overall process of autophagy, as well as individual steps, from yeast to human. The majority of the review considers methods that apply to macroautophagy; however, we also consider alternative types of degradation including chaperone-mediated autophagy and microautophagy. This information is meant to provide a resource for newcomers as well os o stimulus for experienced researchers who may be prompted to develop additional assays to examine autophagy-related pathways.

Original languageEnglish (US)
Pages (from-to)181-206
Number of pages26
JournalAutophagy
Volume3
Issue number3
DOIs
StatePublished - Jan 1 2007

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Keywords

  • Assay
  • Lysosome
  • Protein degradation
  • Protein targeting
  • Trafficking
  • Vacuole

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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