Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

Joseph V. Raimondo, Uwe Heinemann, Marco de Curtis, Howard P. Goodkin, Chris G. Dulla, Damir Janigro, Akio Ikeda, Chou Ching K. Lin, Premysl Jiruska, Aristea S. Galanopoulou, Christophe Bernard

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.

Original languageEnglish (US)
Pages (from-to)40-52
Number of pages13
JournalEpilepsia
Volume58
DOIs
StatePublished - Nov 2017

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Keywords

  • Animal selection and killing
  • Brain slice preparation
  • Electrophysiological recording methods
  • In vitro models of seizures
  • Recording solution composition

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Raimondo, J. V., Heinemann, U., de Curtis, M., Goodkin, H. P., Dulla, C. G., Janigro, D., Ikeda, A., Lin, C. C. K., Jiruska, P., Galanopoulou, A. S., & Bernard, C. (2017). Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE. Epilepsia, 58, 40-52. https://doi.org/10.1111/epi.13901