In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.
- Animal selection and killing
- Brain slice preparation
- Electrophysiological recording methods
- In vitro models of seizures
- Recording solution composition
ASJC Scopus subject areas
- Clinical Neurology