Methodologic recommendations and possible interpretations of video-EEG recordings in immature rodents used as experimental controls: A TASK1-WG2 report of the ILAE/AES Joint Translational Task Force

Ozlem Akman, Yogendra H. Raol, Stéphane Auvin, Miguel A. Cortez, Hana Kubova, Marco de Curtis, Akio Ikeda, F. Edward Dudek, Aristea S. Galanopoulou

Research output: Contribution to journalArticle

Abstract

The use of immature rodents to study physiologic aspects of cortical development requires high-quality recordings electroencephalography (EEG) with simultaneous video recording (vEEG) of behavior. Normative developmental vEEG data in control animals are fundamental for the study of abnormal background activity in animal models of seizures or other neurologic disorders. Electrical recordings from immature, freely behaving rodents can be particularly difficult because of the small size of immature rodents, their thin and soft skull, interference with the recording apparatus by the dam, and other technical challenges. In this report of the TASK1 Working Group 2 (WG2) of the International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force, we provide suggestions that aim to optimize future vEEG recordings from immature rodents, as well as their interpretation. We focus on recordings from immature rodents younger than 30 days old used as experimental controls, because the quality and correct interpretation of such recordings is important when interpreting the vEEG results of animals serving as models of neurologic disorders. We discuss the technical aspects of such recordings and compare tethered versus wireless approaches. We also summarize the appearance of common artifacts and various patterns of electrical activity seen in young rodents used as controls as a function of behavioral state, age, and (where known) sex and strain. The information herein will hopefully help improve the methodology of vEEG recordings from immature rodents and may lead to results and interpretations that are more consistent across studies from different laboratories.

Original languageEnglish (US)
Pages (from-to)437-459
Number of pages23
JournalEpilepsia Open
Volume3
Issue number4
DOIs
StatePublished - Dec 1 2018

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Video Recording
Electroencephalography
Rodentia
Epilepsy
Nervous System Diseases
Advisory Committees
Skull
Quality Control
Artifacts
Seizures
Animal Models
Joints

Keywords

  • Anesthesia
  • Awake
  • Cortical
  • Minimum standards
  • Mouse
  • Ontogeny
  • Postnatal
  • Rat
  • Sleep
  • Spectral analysis
  • Spindles
  • Stereotaxic
  • Subcortical
  • vEEG

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Methodologic recommendations and possible interpretations of video-EEG recordings in immature rodents used as experimental controls : A TASK1-WG2 report of the ILAE/AES Joint Translational Task Force. / Akman, Ozlem; Raol, Yogendra H.; Auvin, Stéphane; Cortez, Miguel A.; Kubova, Hana; de Curtis, Marco; Ikeda, Akio; Dudek, F. Edward; Galanopoulou, Aristea S.

In: Epilepsia Open, Vol. 3, No. 4, 01.12.2018, p. 437-459.

Research output: Contribution to journalArticle

Akman, Ozlem ; Raol, Yogendra H. ; Auvin, Stéphane ; Cortez, Miguel A. ; Kubova, Hana ; de Curtis, Marco ; Ikeda, Akio ; Dudek, F. Edward ; Galanopoulou, Aristea S. / Methodologic recommendations and possible interpretations of video-EEG recordings in immature rodents used as experimental controls : A TASK1-WG2 report of the ILAE/AES Joint Translational Task Force. In: Epilepsia Open. 2018 ; Vol. 3, No. 4. pp. 437-459.
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