Abstract
The use of immature rodents to study physiologic aspects of cortical development requires high-quality recordings electroencephalography (EEG) with simultaneous video recording (vEEG) of behavior. Normative developmental vEEG data in control animals are fundamental for the study of abnormal background activity in animal models of seizures or other neurologic disorders. Electrical recordings from immature, freely behaving rodents can be particularly difficult because of the small size of immature rodents, their thin and soft skull, interference with the recording apparatus by the dam, and other technical challenges. In this report of the TASK1 Working Group 2 (WG2) of the International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force, we provide suggestions that aim to optimize future vEEG recordings from immature rodents, as well as their interpretation. We focus on recordings from immature rodents younger than 30 days old used as experimental controls, because the quality and correct interpretation of such recordings is important when interpreting the vEEG results of animals serving as models of neurologic disorders. We discuss the technical aspects of such recordings and compare tethered versus wireless approaches. We also summarize the appearance of common artifacts and various patterns of electrical activity seen in young rodents used as controls as a function of behavioral state, age, and (where known) sex and strain. The information herein will hopefully help improve the methodology of vEEG recordings from immature rodents and may lead to results and interpretations that are more consistent across studies from different laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 437-459 |
| Number of pages | 23 |
| Journal | Epilepsia Open |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 2018 |
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Keywords
- Anesthesia
- Awake
- Cortical
- Minimum standards
- Mouse
- Ontogeny
- Postnatal
- Rat
- Sleep
- Spectral analysis
- Spindles
- Stereotaxic
- Subcortical
- vEEG
ASJC Scopus subject areas
- Clinical Neurology
- Neurology
Cite this
Methodologic recommendations and possible interpretations of video-EEG recordings in immature rodents used as experimental controls : A TASK1-WG2 report of the ILAE/AES Joint Translational Task Force. / Akman, Ozlem; Raol, Yogendra H.; Auvin, Stéphane; Cortez, Miguel A.; Kubova, Hana; de Curtis, Marco; Ikeda, Akio; Dudek, F. Edward; Galanopoulou, Aristea S.
In: Epilepsia Open, Vol. 3, No. 4, 01.12.2018, p. 437-459.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Methodologic recommendations and possible interpretations of video-EEG recordings in immature rodents used as experimental controls
T2 - A TASK1-WG2 report of the ILAE/AES Joint Translational Task Force
AU - Akman, Ozlem
AU - Raol, Yogendra H.
AU - Auvin, Stéphane
AU - Cortez, Miguel A.
AU - Kubova, Hana
AU - de Curtis, Marco
AU - Ikeda, Akio
AU - Dudek, F. Edward
AU - Galanopoulou, Aristea S.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The use of immature rodents to study physiologic aspects of cortical development requires high-quality recordings electroencephalography (EEG) with simultaneous video recording (vEEG) of behavior. Normative developmental vEEG data in control animals are fundamental for the study of abnormal background activity in animal models of seizures or other neurologic disorders. Electrical recordings from immature, freely behaving rodents can be particularly difficult because of the small size of immature rodents, their thin and soft skull, interference with the recording apparatus by the dam, and other technical challenges. In this report of the TASK1 Working Group 2 (WG2) of the International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force, we provide suggestions that aim to optimize future vEEG recordings from immature rodents, as well as their interpretation. We focus on recordings from immature rodents younger than 30 days old used as experimental controls, because the quality and correct interpretation of such recordings is important when interpreting the vEEG results of animals serving as models of neurologic disorders. We discuss the technical aspects of such recordings and compare tethered versus wireless approaches. We also summarize the appearance of common artifacts and various patterns of electrical activity seen in young rodents used as controls as a function of behavioral state, age, and (where known) sex and strain. The information herein will hopefully help improve the methodology of vEEG recordings from immature rodents and may lead to results and interpretations that are more consistent across studies from different laboratories.
AB - The use of immature rodents to study physiologic aspects of cortical development requires high-quality recordings electroencephalography (EEG) with simultaneous video recording (vEEG) of behavior. Normative developmental vEEG data in control animals are fundamental for the study of abnormal background activity in animal models of seizures or other neurologic disorders. Electrical recordings from immature, freely behaving rodents can be particularly difficult because of the small size of immature rodents, their thin and soft skull, interference with the recording apparatus by the dam, and other technical challenges. In this report of the TASK1 Working Group 2 (WG2) of the International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force, we provide suggestions that aim to optimize future vEEG recordings from immature rodents, as well as their interpretation. We focus on recordings from immature rodents younger than 30 days old used as experimental controls, because the quality and correct interpretation of such recordings is important when interpreting the vEEG results of animals serving as models of neurologic disorders. We discuss the technical aspects of such recordings and compare tethered versus wireless approaches. We also summarize the appearance of common artifacts and various patterns of electrical activity seen in young rodents used as controls as a function of behavioral state, age, and (where known) sex and strain. The information herein will hopefully help improve the methodology of vEEG recordings from immature rodents and may lead to results and interpretations that are more consistent across studies from different laboratories.
KW - Anesthesia
KW - Awake
KW - Cortical
KW - Minimum standards
KW - Mouse
KW - Ontogeny
KW - Postnatal
KW - Rat
KW - Sleep
KW - Spectral analysis
KW - Spindles
KW - Stereotaxic
KW - Subcortical
KW - vEEG
UR - http://www.scopus.com/inward/record.url?scp=85063922773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063922773&partnerID=8YFLogxK
U2 - 10.1002/epi4.12262
DO - 10.1002/epi4.12262
M3 - Article
AN - SCOPUS:85063922773
VL - 3
SP - 437
EP - 459
JO - Epilepsia Open
JF - Epilepsia Open
SN - 2470-9239
IS - 4
ER -