Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model

Sanchita P. Ghosh, Rajbir Singh, Kushal Chakraborty, Shilpa Kulkarni, Arushi Uppal, Yue Luo, Prabhjit Kaur, Rupak Pathak, K. Sree Kumar, Martin Hauer-Jensen, Amrita K. Cheema

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.

Original languageEnglish (US)
Pages (from-to)723-731
Number of pages9
JournalMolecular BioSystems
Volume9
Issue number4
DOIs
StatePublished - Apr 2013
Externally publishedYes

Fingerprint

Metabolomics
Whole-Body Irradiation
Ionizing Radiation
Radiation Injuries
Wounds and Injuries
Liquid Chromatography
Mass Spectrometry
Biomarkers
Urine
Radiation
Phenotype
Gene Expression
Serum
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Ghosh, S. P., Singh, R., Chakraborty, K., Kulkarni, S., Uppal, A., Luo, Y., ... Cheema, A. K. (2013). Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model. Molecular BioSystems, 9(4), 723-731. https://doi.org/10.1039/c3mb25454b

Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model. / Ghosh, Sanchita P.; Singh, Rajbir; Chakraborty, Kushal; Kulkarni, Shilpa; Uppal, Arushi; Luo, Yue; Kaur, Prabhjit; Pathak, Rupak; Kumar, K. Sree; Hauer-Jensen, Martin; Cheema, Amrita K.

In: Molecular BioSystems, Vol. 9, No. 4, 04.2013, p. 723-731.

Research output: Contribution to journalArticle

Ghosh, SP, Singh, R, Chakraborty, K, Kulkarni, S, Uppal, A, Luo, Y, Kaur, P, Pathak, R, Kumar, KS, Hauer-Jensen, M & Cheema, AK 2013, 'Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model', Molecular BioSystems, vol. 9, no. 4, pp. 723-731. https://doi.org/10.1039/c3mb25454b
Ghosh, Sanchita P. ; Singh, Rajbir ; Chakraborty, Kushal ; Kulkarni, Shilpa ; Uppal, Arushi ; Luo, Yue ; Kaur, Prabhjit ; Pathak, Rupak ; Kumar, K. Sree ; Hauer-Jensen, Martin ; Cheema, Amrita K. / Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model. In: Molecular BioSystems. 2013 ; Vol. 9, No. 4. pp. 723-731.
@article{91980c4537e947cb9fddd792919595fd,
title = "Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model",
abstract = "Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.",
author = "Ghosh, {Sanchita P.} and Rajbir Singh and Kushal Chakraborty and Shilpa Kulkarni and Arushi Uppal and Yue Luo and Prabhjit Kaur and Rupak Pathak and Kumar, {K. Sree} and Martin Hauer-Jensen and Cheema, {Amrita K.}",
year = "2013",
month = "4",
doi = "10.1039/c3mb25454b",
language = "English (US)",
volume = "9",
pages = "723--731",
journal = "Molecular BioSystems",
issn = "1742-206X",
publisher = "Royal Society of Chemistry",
number = "4",

}

TY - JOUR

T1 - Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model

AU - Ghosh, Sanchita P.

AU - Singh, Rajbir

AU - Chakraborty, Kushal

AU - Kulkarni, Shilpa

AU - Uppal, Arushi

AU - Luo, Yue

AU - Kaur, Prabhjit

AU - Pathak, Rupak

AU - Kumar, K. Sree

AU - Hauer-Jensen, Martin

AU - Cheema, Amrita K.

PY - 2013/4

Y1 - 2013/4

N2 - Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.

AB - Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.

UR - http://www.scopus.com/inward/record.url?scp=84874828100&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874828100&partnerID=8YFLogxK

U2 - 10.1039/c3mb25454b

DO - 10.1039/c3mb25454b

M3 - Article

C2 - 23403731

AN - SCOPUS:84874828100

VL - 9

SP - 723

EP - 731

JO - Molecular BioSystems

JF - Molecular BioSystems

SN - 1742-206X

IS - 4

ER -