Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model

Sanchita P. Ghosh; Rajbir Singh; Kushal Chakraborty; Shilpa Kulkarni; Arushi Uppal; Yue Luo; Prabhjit Kaur; Rupak Pathak; K. Sree Kumar; Martin Hauer-Jensen; Amrita K. Cheema

doi:10.1039/c3mb25454b

Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model

Sanchita P. Ghosh, Rajbir Singh, Kushal Chakraborty, Shilpa Kulkarni, Arushi Uppal, Yue Luo, Prabhjit Kaur, Rupak Pathak, K. Sree Kumar, Martin Hauer-Jensen, Amrita K. Cheema

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.

Original language	English (US)
Pages (from-to)	723-731
Number of pages	9
Journal	Molecular BioSystems
Volume	9
Issue number	4
DOIs	https://doi.org/10.1039/c3mb25454b
State	Published - Apr 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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abstract = "Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.",

author = "Ghosh, {Sanchita P.} and Rajbir Singh and Kushal Chakraborty and Shilpa Kulkarni and Arushi Uppal and Yue Luo and Prabhjit Kaur and Rupak Pathak and Kumar, {K. Sree} and Martin Hauer-Jensen and Cheema, {Amrita K.}",

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AU - Ghosh, Sanchita P.

AU - Singh, Rajbir

AU - Chakraborty, Kushal

AU - Kulkarni, Shilpa

AU - Uppal, Arushi

AU - Luo, Yue

AU - Kaur, Prabhjit

AU - Pathak, Rupak

AU - Kumar, K. Sree

AU - Hauer-Jensen, Martin

AU - Cheema, Amrita K.

PY - 2013/4

Y1 - 2013/4

N2 - Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.

AB - Exposure to ionizing radiation (IR) elicits a set of complex biological responses involving gene expression and protein turnover that ultimately manifest as dysregulation of metabolic processes representing the cellular phenotype. Although radiation biomarkers have been reported in urine and serum, they are not informative about IR mediated tissue or organ specific injury. In the present study we report IR induced metabolic changes in gastrointestinal (GI) tissue of CD2F1 mice using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry. Post-radiation GI injury is a critical determinant of survival after exposure to IR. Our results show a distinct dose and time dependent response to GI tissue injury.

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Metabolomic changes in gastrointestinal tissues after whole body radiation in a murine model

Abstract

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