Metabolite profiling of Panax notoginseng using UPLC-ESI-MS

Mo Dan, Mingming Su, Xianfu Gao, Tie Zhao, Aihua Zhao, Guoxiang Xie, Yunping Qiu, Mingmei Zhou, Zhong Liu, Wei Jia

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

The metabolite profiling of different parts of Panax notoginseng was carried out using rapid ultra-performance liquid chromatography-electrospray ionization mass spectrometry (UPLC-ESI-MS) and multivariate statistical analysis. Principal component analysis (PCA) of the UPLC-ESI-MS data showed a clear separation of compositions among the flower buds, roots and rhizomes of P. notoginseng. The saponins accounting for such variations were identified through the corresponding loadings weights and were further verified by accurate mass, tandem mass and retention times of available standard saponins using UPLC quadrupole time-of-flight mass spectrometer (UPLC-QtofMS). Finally, the influential factors of different metabolic phenotypes of P. notoginseng was elucidated. The currently proposed UPLC-ESI-MS/MS analytical method coupled with multivariate statistical analysis can be further utilized to evaluate chemical components obtained from different parts of the plant and/or the plant of different geographical locations, thereby classifying the medicinal plant resources and potentially elucidating the mechanism of inherent phytochemical diversity.

Original languageEnglish (US)
Pages (from-to)2237-2244
Number of pages8
JournalPhytochemistry
Volume69
Issue number11
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

Keywords

  • Electrospray ionization (ESI)
  • Metabolite profiling
  • Multivariate statistical analysis
  • Panax notoginseng
  • Quadrupole time-of-flight mass spectrometer (QtofMS)
  • Saponin
  • Ultra-performance liquid chromatography (UPLC)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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  • Cite this

    Dan, M., Su, M., Gao, X., Zhao, T., Zhao, A., Xie, G., Qiu, Y., Zhou, M., Liu, Z., & Jia, W. (2008). Metabolite profiling of Panax notoginseng using UPLC-ESI-MS. Phytochemistry, 69(11), 2237-2244. https://doi.org/10.1016/j.phytochem.2008.04.015