Mercury 203 distribution in pregnant and nonpregnant rats following systemic infusions with thiol-containing amino acids

Michael Aschner, W. Clarkson Th.

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Near-term pregnant (gestational day 17) and nonpregnant Long-Evans female rats were continuously infused into the external jugular vein with 0.1 mmole/hour L-cysteine, 0.1 mmole/hour L-leucine, or saline. At 24, 48, and 72 hours, 50 μmole/hour [203Hg]-MeHgCl was administered over 1 hour. Total 203Hg body burden, brain, kidney, liver, and blood 203Hg concentrations were determined at 96 hours by gamma scintillation spectrometry. Despite significantly greater 203Hg whole body retention in the pregnant animals 203Hg concentrations in blood, brain, kidney, and liver were higher in nonpregnant rats. In addition, brain 203Hg concentrations in both pregnant and virgin rats were significantly higher in L-cysteine-treated rats compared with controls. These results suggest that the fetus may act as a 'sink' for MeHg, thus decreasing 203Hg concentrations in maternal blood, brain, kidney, and liver. Furthermore, the data indicate that brain uptake of methylmercury in both pregnant and nonpregnant rats is enhanced by chronic L-cysteine infusion, lending support to the hypothesis that methylmercury in the rat may be translocated across the blood-brain barrier by the neutral amino acid carrier transport system.

Original languageEnglish (US)
Pages (from-to)321-328
Number of pages8
JournalTeratology
Volume36
Issue number3
StatePublished - 1987
Externally publishedYes

Fingerprint

Mercury
Sulfhydryl Compounds
Rats
Brain
Amino Acids
Cysteine
Liver
Blood
Kidney
Neutral Amino Acid Transport Systems
Gamma Spectrometry
Body Burden
Long Evans Rats
Neutral Amino Acids
Jugular Veins
Blood-Brain Barrier
Carrier transport
Leucine
Scintillation
Fetus

ASJC Scopus subject areas

  • Developmental Biology
  • Health, Toxicology and Mutagenesis
  • Embryology
  • Toxicology

Cite this

Mercury 203 distribution in pregnant and nonpregnant rats following systemic infusions with thiol-containing amino acids. / Aschner, Michael; Clarkson Th., W.

In: Teratology, Vol. 36, No. 3, 1987, p. 321-328.

Research output: Contribution to journalArticle

@article{2c67b9648f7042dd82a07d57795dbbb4,
title = "Mercury 203 distribution in pregnant and nonpregnant rats following systemic infusions with thiol-containing amino acids",
abstract = "Near-term pregnant (gestational day 17) and nonpregnant Long-Evans female rats were continuously infused into the external jugular vein with 0.1 mmole/hour L-cysteine, 0.1 mmole/hour L-leucine, or saline. At 24, 48, and 72 hours, 50 μmole/hour [203Hg]-MeHgCl was administered over 1 hour. Total 203Hg body burden, brain, kidney, liver, and blood 203Hg concentrations were determined at 96 hours by gamma scintillation spectrometry. Despite significantly greater 203Hg whole body retention in the pregnant animals 203Hg concentrations in blood, brain, kidney, and liver were higher in nonpregnant rats. In addition, brain 203Hg concentrations in both pregnant and virgin rats were significantly higher in L-cysteine-treated rats compared with controls. These results suggest that the fetus may act as a 'sink' for MeHg, thus decreasing 203Hg concentrations in maternal blood, brain, kidney, and liver. Furthermore, the data indicate that brain uptake of methylmercury in both pregnant and nonpregnant rats is enhanced by chronic L-cysteine infusion, lending support to the hypothesis that methylmercury in the rat may be translocated across the blood-brain barrier by the neutral amino acid carrier transport system.",
author = "Michael Aschner and {Clarkson Th.}, W.",
year = "1987",
language = "English (US)",
volume = "36",
pages = "321--328",
journal = "Teratology",
issn = "1542-0752",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Mercury 203 distribution in pregnant and nonpregnant rats following systemic infusions with thiol-containing amino acids

AU - Aschner, Michael

AU - Clarkson Th., W.

PY - 1987

Y1 - 1987

N2 - Near-term pregnant (gestational day 17) and nonpregnant Long-Evans female rats were continuously infused into the external jugular vein with 0.1 mmole/hour L-cysteine, 0.1 mmole/hour L-leucine, or saline. At 24, 48, and 72 hours, 50 μmole/hour [203Hg]-MeHgCl was administered over 1 hour. Total 203Hg body burden, brain, kidney, liver, and blood 203Hg concentrations were determined at 96 hours by gamma scintillation spectrometry. Despite significantly greater 203Hg whole body retention in the pregnant animals 203Hg concentrations in blood, brain, kidney, and liver were higher in nonpregnant rats. In addition, brain 203Hg concentrations in both pregnant and virgin rats were significantly higher in L-cysteine-treated rats compared with controls. These results suggest that the fetus may act as a 'sink' for MeHg, thus decreasing 203Hg concentrations in maternal blood, brain, kidney, and liver. Furthermore, the data indicate that brain uptake of methylmercury in both pregnant and nonpregnant rats is enhanced by chronic L-cysteine infusion, lending support to the hypothesis that methylmercury in the rat may be translocated across the blood-brain barrier by the neutral amino acid carrier transport system.

AB - Near-term pregnant (gestational day 17) and nonpregnant Long-Evans female rats were continuously infused into the external jugular vein with 0.1 mmole/hour L-cysteine, 0.1 mmole/hour L-leucine, or saline. At 24, 48, and 72 hours, 50 μmole/hour [203Hg]-MeHgCl was administered over 1 hour. Total 203Hg body burden, brain, kidney, liver, and blood 203Hg concentrations were determined at 96 hours by gamma scintillation spectrometry. Despite significantly greater 203Hg whole body retention in the pregnant animals 203Hg concentrations in blood, brain, kidney, and liver were higher in nonpregnant rats. In addition, brain 203Hg concentrations in both pregnant and virgin rats were significantly higher in L-cysteine-treated rats compared with controls. These results suggest that the fetus may act as a 'sink' for MeHg, thus decreasing 203Hg concentrations in maternal blood, brain, kidney, and liver. Furthermore, the data indicate that brain uptake of methylmercury in both pregnant and nonpregnant rats is enhanced by chronic L-cysteine infusion, lending support to the hypothesis that methylmercury in the rat may be translocated across the blood-brain barrier by the neutral amino acid carrier transport system.

UR - http://www.scopus.com/inward/record.url?scp=0023581917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023581917&partnerID=8YFLogxK

M3 - Article

C2 - 3424221

AN - SCOPUS:0023581917

VL - 36

SP - 321

EP - 328

JO - Teratology

JF - Teratology

SN - 1542-0752

IS - 3

ER -