Mechanism of interleukin-1β stimulation of human amnion prostaglandin biosynthesis: Mediation via a novel inducible cyclooxygenase

M. D. Mitchell, S. S. Edwin, S. Lundin-Schiller, R. M. Silver, D. Smotkin, M. S. Trautman

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

We have evaluated the mechanism by which interleukin-1β (IL-1β) increases amnion cell PGE2 production in a concentration-dependent manner. IL-1β-stimulated amnion cell PGE2 biosynthesis was time-dependent, and significant stimulation occurred within 2 h of incubation. IL-1β stimulation occurred in the presence of added arachidonic acid but was abrogated by treatment with cycloheximide and actinomycin D. Amnion cells treated with IL-1β recovered rapidly from aspirin pretreatment suggesting an action on fatty acid cyclooxygenase (COX). Increased amounts of COX protein were demonstrated by Western blot analysis within 2 h of IL-1β treatment of amnion cells. Northern blot analysis using a probe specific for a novel form of COX (COX-II) showed an increase in mRNA for this COX within 30 min. This finding using a homologous detection system and human cells of fetal origin in primary culture provides strong support for a physiological role for COX-II in man.

Original languageEnglish (US)
Pages (from-to)615-625
Number of pages11
JournalPlacenta
Volume14
Issue number6
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

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