Mechanism of interleukin-1β stimulation of human amnion prostaglandin biosynthesis

Mediation via a novel inducible cyclooxygenase

M. D. Mitchell, S. S. Edwin, S. Lundin-Schiller, R. M. Silver, David Smotkin, M. S. Trautman

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

We have evaluated the mechanism by which interleukin-1β (IL-1β) increases amnion cell PGE2 production in a concentration-dependent manner. IL-1β-stimulated amnion cell PGE2 biosynthesis was time-dependent, and significant stimulation occurred within 2 h of incubation. IL-1β stimulation occurred in the presence of added arachidonic acid but was abrogated by treatment with cycloheximide and actinomycin D. Amnion cells treated with IL-1β recovered rapidly from aspirin pretreatment suggesting an action on fatty acid cyclooxygenase (COX). Increased amounts of COX protein were demonstrated by Western blot analysis within 2 h of IL-1β treatment of amnion cells. Northern blot analysis using a probe specific for a novel form of COX (COX-II) showed an increase in mRNA for this COX within 30 min. This finding using a homologous detection system and human cells of fetal origin in primary culture provides strong support for a physiological role for COX-II in man.

Original languageEnglish (US)
Pages (from-to)615-625
Number of pages11
JournalPlacenta
Volume14
Issue number6
StatePublished - 1993
Externally publishedYes

Fingerprint

Amnion
Prostaglandin-Endoperoxide Synthases
Interleukin-1
Prostaglandins
Dinoprostone
Dactinomycin
Cycloheximide
Arachidonic Acid
Northern Blotting
Aspirin
Fatty Acids
Western Blotting
Messenger RNA
Therapeutics
Proteins

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Mitchell, M. D., Edwin, S. S., Lundin-Schiller, S., Silver, R. M., Smotkin, D., & Trautman, M. S. (1993). Mechanism of interleukin-1β stimulation of human amnion prostaglandin biosynthesis: Mediation via a novel inducible cyclooxygenase. Placenta, 14(6), 615-625.

Mechanism of interleukin-1β stimulation of human amnion prostaglandin biosynthesis : Mediation via a novel inducible cyclooxygenase. / Mitchell, M. D.; Edwin, S. S.; Lundin-Schiller, S.; Silver, R. M.; Smotkin, David; Trautman, M. S.

In: Placenta, Vol. 14, No. 6, 1993, p. 615-625.

Research output: Contribution to journalArticle

Mitchell, MD, Edwin, SS, Lundin-Schiller, S, Silver, RM, Smotkin, D & Trautman, MS 1993, 'Mechanism of interleukin-1β stimulation of human amnion prostaglandin biosynthesis: Mediation via a novel inducible cyclooxygenase', Placenta, vol. 14, no. 6, pp. 615-625.
Mitchell, M. D. ; Edwin, S. S. ; Lundin-Schiller, S. ; Silver, R. M. ; Smotkin, David ; Trautman, M. S. / Mechanism of interleukin-1β stimulation of human amnion prostaglandin biosynthesis : Mediation via a novel inducible cyclooxygenase. In: Placenta. 1993 ; Vol. 14, No. 6. pp. 615-625.
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