Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments

Katrina M. Wisdom, Kolade Adebowale, Julie Chang, Joanna Y. Lee, Sungmin Nam, Rajiv Desai, Ninna Struck Rossen, Marjan Rafat, Robert B. West, Louis Hodgson, Ovijit Chaudhuri

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Studies of cancer cell migration have found two modes: one that is protease-independent, requiring micron-sized pores or channels for cells to squeeze through, and one that is protease-dependent, relevant for confining nanoporous matrices such as basement membranes (BMs). However, many extracellular matrices exhibit viscoelasticity and mechanical plasticity, irreversibly deforming in response to force, so that pore size may be malleable. Here we report the impact of matrix plasticity on migration. We develop nanoporous and BM ligand-presenting interpenetrating network (IPN) hydrogels in which plasticity could be modulated independent of stiffness. Strikingly, cells in high plasticity IPNs carry out protease-independent migration through the IPNs. Mechanistically, cells in high plasticity IPNs extend invadopodia protrusions to mechanically and plastically open up micron-sized channels and then migrate through them. These findings uncover a new mode of protease-independent migration, in which cells can migrate through confining matrix if it exhibits sufficient mechanical plasticity.

Original languageEnglish (US)
Article number4144
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

plastic properties
confining
Cell Movement
Plasticity
protease
Peptide Hydrolases
cancer
Cells
matrices
Basement Membrane
Neoplasms
cells
basements
Hydrogels
membranes
Extracellular Matrix
porosity
Interpenetrating polymer networks
viscoelasticity
Viscoelasticity

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Wisdom, K. M., Adebowale, K., Chang, J., Lee, J. Y., Nam, S., Desai, R., ... Chaudhuri, O. (2018). Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments. Nature Communications, 9(1), [4144]. https://doi.org/10.1038/s41467-018-06641-z

Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments. / Wisdom, Katrina M.; Adebowale, Kolade; Chang, Julie; Lee, Joanna Y.; Nam, Sungmin; Desai, Rajiv; Rossen, Ninna Struck; Rafat, Marjan; West, Robert B.; Hodgson, Louis; Chaudhuri, Ovijit.

In: Nature Communications, Vol. 9, No. 1, 4144, 01.12.2018.

Research output: Contribution to journalArticle

Wisdom, KM, Adebowale, K, Chang, J, Lee, JY, Nam, S, Desai, R, Rossen, NS, Rafat, M, West, RB, Hodgson, L & Chaudhuri, O 2018, 'Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments', Nature Communications, vol. 9, no. 1, 4144. https://doi.org/10.1038/s41467-018-06641-z
Wisdom, Katrina M. ; Adebowale, Kolade ; Chang, Julie ; Lee, Joanna Y. ; Nam, Sungmin ; Desai, Rajiv ; Rossen, Ninna Struck ; Rafat, Marjan ; West, Robert B. ; Hodgson, Louis ; Chaudhuri, Ovijit. / Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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