Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila

L. A. Herndon, T. Chapman, J. M. Kalb, S. Lewin, L. Partridge, M. F. Wolfner

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Drosophila melanogaster males transfer accessory gland proteins, as part of their seminal fluid, to females during each mating. Since accessory gland proteins are important for male reproductive success, it is important that the male replenish the proteins he transferred during mating. Previous studies had shown that mating induces the resynthesis of accessory gland proteins, but since mating includes a set of stereotyped behavior patterns as well as the act of copulation, it was not known which aspect of the mating process induces accessory gland protein synthesis. By exposing males to females whose ovipositors had been sealed shut, we have shown that resynthesis of accessory gland proteins occurs only when seminal fluid is transferred to females. By applying juvenile hormone or 20-hydroxyecdysone topically to the cuticle of male flies, we showed that these hormones can act in vivo to stimulate the synthesis of accessory gland proteins to levels similar to those observed after mating.

Original languageEnglish (US)
Pages (from-to)1117-1123
Number of pages7
JournalJournal of Insect Physiology
Volume43
Issue number12
DOIs
StatePublished - Nov 1997
Externally publishedYes

Fingerprint

Drosophila
Gene Expression
gene expression
Proteins
proteins
stereotyped behavior
ovipositor
Ecdysterone
ecdysterone
Stereotyped Behavior
Copulation
Juvenile Hormones
juvenile hormones
copulation
Drosophila melanogaster
Diptera
protein synthesis
hormones
Hormones
synthesis

Keywords

  • Accessory glands
  • Drosophila
  • Ecdysone
  • Juvenile hormone
  • Seminal fluid

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Herndon, L. A., Chapman, T., Kalb, J. M., Lewin, S., Partridge, L., & Wolfner, M. F. (1997). Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila. Journal of Insect Physiology, 43(12), 1117-1123. https://doi.org/10.1016/S0022-1910(97)00062-0

Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila. / Herndon, L. A.; Chapman, T.; Kalb, J. M.; Lewin, S.; Partridge, L.; Wolfner, M. F.

In: Journal of Insect Physiology, Vol. 43, No. 12, 11.1997, p. 1117-1123.

Research output: Contribution to journalArticle

Herndon, LA, Chapman, T, Kalb, JM, Lewin, S, Partridge, L & Wolfner, MF 1997, 'Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila', Journal of Insect Physiology, vol. 43, no. 12, pp. 1117-1123. https://doi.org/10.1016/S0022-1910(97)00062-0
Herndon, L. A. ; Chapman, T. ; Kalb, J. M. ; Lewin, S. ; Partridge, L. ; Wolfner, M. F. / Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila. In: Journal of Insect Physiology. 1997 ; Vol. 43, No. 12. pp. 1117-1123.
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