Marine phytoplankton effects on biological activity of human glioma stem cells in vitro

Xiao bing Duan, Fangxia Guan, Xiao hui Deng, Bo Yang, Tian xiang Zhang, Yi Tian, Xiao jun Qiao, Yuan Li, Shuo Liang, Wan wan Zhu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BACKGROUND: Marine phytoplankton (MPPT) has important guiding significance for application to strengthen the research. OBJECTIVE: To investigate the effect of MPPT on biologic activity of brain gliomastem cells (BGSCs) in vitro. METHODS: BGSCs cultured in the enzyme digestion way. The CD133 positive cells were sorted out by flow cytometry, and the 3 rd passage of BGSCs were gathered through sub-culturing. CD133 expression was identified by flow cytometry before and after the effect of MPPT. Immunohistochemisty was used to detect Nestin and glial fibrillary acidic protein (GFAP) expression of adherent cells. The experimental group was added with different concentrations of MPPT, the negative control group was added with just PBS. 4, 6, 8, 10 g/L MPPT was added into the cell culture fluid. The cell cycle of BGSCs was inspected by flow cytometry, and inhibitory effects were detected using a microplate reader respectively at 24, 48, 72 hours later. RESULTS AND CONCLUSION: Compared to those in the control group, BGSCs in the experimental group could not form a compact, and began to be adherent and differentiated gradually observed by an inverted microscope, with the increasing of time and concentration. CD133 expression in BGSCs after the effect of MPPT was reduced obviously. The adherent cells expressed GFAP and Nestin. The cell number in S and G 2/M phase was increased, and that in G 0/G 1 phase was decreased. Growth curve indicated that the proliferation of BGSCs was inhibited obviously, with the increasing of time and concentration (P < 0.05-0.01). Results indicate MPTT can inhibit the proliferation of BGSCs, and promote them differentiate, which shows a dose and time dependent effect.

Original languageEnglish (US)
Pages (from-to)4215-4219
Number of pages5
JournalJournal of Clinical Rehabilitative Tissue Engineering Research
Volume15
Issue number23
DOIs
StatePublished - Jun 2011
Externally publishedYes

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Phytoplankton
Bioactivity
Stem cells
Human Activities
Glioma
Brain
Stem Cells
Flow cytometry
Nestin
Glial Fibrillary Acidic Protein
Cells
Flow Cytometry
Proteins
In Vitro Techniques
Control Groups
Cell culture
Microscopes
Enzymes
Cell Division
Digestion

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biomedical Engineering
  • Transplantation

Cite this

Marine phytoplankton effects on biological activity of human glioma stem cells in vitro. / Duan, Xiao bing; Guan, Fangxia; Deng, Xiao hui; Yang, Bo; Zhang, Tian xiang; Tian, Yi; Qiao, Xiao jun; Li, Yuan; Liang, Shuo; Zhu, Wan wan.

In: Journal of Clinical Rehabilitative Tissue Engineering Research, Vol. 15, No. 23, 06.2011, p. 4215-4219.

Research output: Contribution to journalArticle

Duan, XB, Guan, F, Deng, XH, Yang, B, Zhang, TX, Tian, Y, Qiao, XJ, Li, Y, Liang, S & Zhu, WW 2011, 'Marine phytoplankton effects on biological activity of human glioma stem cells in vitro', Journal of Clinical Rehabilitative Tissue Engineering Research, vol. 15, no. 23, pp. 4215-4219. https://doi.org/10.3969/j.issn.1673-8225.2011.23.009
Duan, Xiao bing ; Guan, Fangxia ; Deng, Xiao hui ; Yang, Bo ; Zhang, Tian xiang ; Tian, Yi ; Qiao, Xiao jun ; Li, Yuan ; Liang, Shuo ; Zhu, Wan wan. / Marine phytoplankton effects on biological activity of human glioma stem cells in vitro. In: Journal of Clinical Rehabilitative Tissue Engineering Research. 2011 ; Vol. 15, No. 23. pp. 4215-4219.
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abstract = "BACKGROUND: Marine phytoplankton (MPPT) has important guiding significance for application to strengthen the research. OBJECTIVE: To investigate the effect of MPPT on biologic activity of brain gliomastem cells (BGSCs) in vitro. METHODS: BGSCs cultured in the enzyme digestion way. The CD133 positive cells were sorted out by flow cytometry, and the 3 rd passage of BGSCs were gathered through sub-culturing. CD133 expression was identified by flow cytometry before and after the effect of MPPT. Immunohistochemisty was used to detect Nestin and glial fibrillary acidic protein (GFAP) expression of adherent cells. The experimental group was added with different concentrations of MPPT, the negative control group was added with just PBS. 4, 6, 8, 10 g/L MPPT was added into the cell culture fluid. The cell cycle of BGSCs was inspected by flow cytometry, and inhibitory effects were detected using a microplate reader respectively at 24, 48, 72 hours later. RESULTS AND CONCLUSION: Compared to those in the control group, BGSCs in the experimental group could not form a compact, and began to be adherent and differentiated gradually observed by an inverted microscope, with the increasing of time and concentration. CD133 expression in BGSCs after the effect of MPPT was reduced obviously. The adherent cells expressed GFAP and Nestin. The cell number in S and G 2/M phase was increased, and that in G 0/G 1 phase was decreased. Growth curve indicated that the proliferation of BGSCs was inhibited obviously, with the increasing of time and concentration (P < 0.05-0.01). Results indicate MPTT can inhibit the proliferation of BGSCs, and promote them differentiate, which shows a dose and time dependent effect.",
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AU - Zhang, Tian xiang

AU - Tian, Yi

AU - Qiao, Xiao jun

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AU - Liang, Shuo

AU - Zhu, Wan wan

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