Marine phytoplankton effects on biological activity of human glioma stem cells in vitro

BACKGROUND: Marine phytoplankton (MPPT) has important guiding significance for application to strengthen the research. OBJECTIVE: To investigate the effect of MPPT on biologic activity of brain gliomastem cells (BGSCs) in vitro. METHODS: BGSCs cultured in the enzyme digestion way. The CD133 positive cells were sorted out by flow cytometry, and the 3 ^rd passage of BGSCs were gathered through sub-culturing. CD133 expression was identified by flow cytometry before and after the effect of MPPT. Immunohistochemisty was used to detect Nestin and glial fibrillary acidic protein (GFAP) expression of adherent cells. The experimental group was added with different concentrations of MPPT, the negative control group was added with just PBS. 4, 6, 8, 10 g/L MPPT was added into the cell culture fluid. The cell cycle of BGSCs was inspected by flow cytometry, and inhibitory effects were detected using a microplate reader respectively at 24, 48, 72 hours later. RESULTS AND CONCLUSION: Compared to those in the control group, BGSCs in the experimental group could not form a compact, and began to be adherent and differentiated gradually observed by an inverted microscope, with the increasing of time and concentration. CD133 expression in BGSCs after the effect of MPPT was reduced obviously. The adherent cells expressed GFAP and Nestin. The cell number in S and G ₂/M phase was increased, and that in G ₀/G ₁ phase was decreased. Growth curve indicated that the proliferation of BGSCs was inhibited obviously, with the increasing of time and concentration (P < 0.05-0.01). Results indicate MPTT can inhibit the proliferation of BGSCs, and promote them differentiate, which shows a dose and time dependent effect.