Mapping T cell epitopes by flow cytometry

Bodo Hoffmeister, Felix Kiecker, Lydia Tesfa, Hans Dieter Volk, Louis J. Picker, Florian Kern

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Epitope mapping by flow cytometry is a very modern approach that not only identifies T-cell epitopes but simultaneously allows for detailed analysis of the responding T-cell subsets including lineage, activation marker expression, and other markers of interest. The most frequently used approach is based on the identification of intracellular cytokines in secretion-inhibited activated T cells following stimulation with peptides or peptide pools. A more recently developed assay analyzes T-cell proliferation by measuring the decrease in carboxyfluorescein diacetate succinimidyl ester staining in proliferated cells. This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays.

Original languageEnglish (US)
Pages (from-to)270-281
Number of pages12
JournalMethods
Volume29
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

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T-Lymphocyte Epitopes
Flow cytometry
T-cells
Flow Cytometry
Peptides
Assays
Epitope Mapping
T-Lymphocytes
Cell proliferation
T-Lymphocyte Subsets
Epitopes
Chemical activation
Cell Proliferation
Staining and Labeling
Cytokines

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Hoffmeister, B., Kiecker, F., Tesfa, L., Volk, H. D., Picker, L. J., & Kern, F. (2003). Mapping T cell epitopes by flow cytometry. Methods, 29(3), 270-281. https://doi.org/10.1016/S1046-2023(02)00349-3

Mapping T cell epitopes by flow cytometry. / Hoffmeister, Bodo; Kiecker, Felix; Tesfa, Lydia; Volk, Hans Dieter; Picker, Louis J.; Kern, Florian.

In: Methods, Vol. 29, No. 3, 01.03.2003, p. 270-281.

Research output: Contribution to journalArticle

Hoffmeister, B, Kiecker, F, Tesfa, L, Volk, HD, Picker, LJ & Kern, F 2003, 'Mapping T cell epitopes by flow cytometry', Methods, vol. 29, no. 3, pp. 270-281. https://doi.org/10.1016/S1046-2023(02)00349-3
Hoffmeister B, Kiecker F, Tesfa L, Volk HD, Picker LJ, Kern F. Mapping T cell epitopes by flow cytometry. Methods. 2003 Mar 1;29(3):270-281. https://doi.org/10.1016/S1046-2023(02)00349-3
Hoffmeister, Bodo ; Kiecker, Felix ; Tesfa, Lydia ; Volk, Hans Dieter ; Picker, Louis J. ; Kern, Florian. / Mapping T cell epitopes by flow cytometry. In: Methods. 2003 ; Vol. 29, No. 3. pp. 270-281.
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