Mapping T cell epitopes by flow cytometry

Bodo Hoffmeister, Felix Kiecker, Lydia Tesfa, Hans Dieter Volk, Louis J. Picker, Florian Kern

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Epitope mapping by flow cytometry is a very modern approach that not only identifies T-cell epitopes but simultaneously allows for detailed analysis of the responding T-cell subsets including lineage, activation marker expression, and other markers of interest. The most frequently used approach is based on the identification of intracellular cytokines in secretion-inhibited activated T cells following stimulation with peptides or peptide pools. A more recently developed assay analyzes T-cell proliferation by measuring the decrease in carboxyfluorescein diacetate succinimidyl ester staining in proliferated cells. This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays.

Original languageEnglish (US)
Pages (from-to)270-281
Number of pages12
JournalMethods
Volume29
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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    Hoffmeister, B., Kiecker, F., Tesfa, L., Volk, H. D., Picker, L. J., & Kern, F. (2003). Mapping T cell epitopes by flow cytometry. Methods, 29(3), 270-281. https://doi.org/10.1016/S1046-2023(02)00349-3