Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI

Takashi Watanabe; Jelena Radulovic; Susann Boretius; Jens Frahm; Thomas Michaelis

doi:10.1016/j.mri.2005.10.034

Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI

Takashi Watanabe, Jelena Radulovic, Susann Boretius, Jens Frahm, Thomas Michaelis

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

This magnetic resonance imaging (MRI) study describes mapping of the habenulo-interpeduncular pathway in living mice based on manganese-induced contrast. Six hours after intracerebroventricular microinjection of MnCl ₂, T1-weighted 3D MRI (2.35 T) at 117 μm isotropic resolution revealed a continuous pattern of anterograde labeling from the habenula via the fasciculus retroflexus to the interpeduncular nucleus. Alternatively, the less invasive systemic administration of MnCl₂ allowed for monitoring of the dynamic uptake pattern of respective neural components with even higher reproducibility across animals. Time courses covered the range from 42 min to 24 h after injection. In conclusion, manganese-enhanced MRI may open new ways for functional assessments of the habenulo-interpeduncular system in animal models with cognitive impairment.

Original language	English (US)
Pages (from-to)	209-215
Number of pages	7
Journal	Magnetic Resonance Imaging
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.mri.2005.10.034
State	Published - Apr 2006
Externally published	Yes

Keywords

Habenula
Magnetic resonance imaging
Manganese
Mice
Neural pathways

ASJC Scopus subject areas

Biophysics
Biomedical Engineering
Radiology Nuclear Medicine and imaging

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10.1016/j.mri.2005.10.034

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title = "Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI",

abstract = "This magnetic resonance imaging (MRI) study describes mapping of the habenulo-interpeduncular pathway in living mice based on manganese-induced contrast. Six hours after intracerebroventricular microinjection of MnCl 2, T1-weighted 3D MRI (2.35 T) at 117 μm isotropic resolution revealed a continuous pattern of anterograde labeling from the habenula via the fasciculus retroflexus to the interpeduncular nucleus. Alternatively, the less invasive systemic administration of MnCl2 allowed for monitoring of the dynamic uptake pattern of respective neural components with even higher reproducibility across animals. Time courses covered the range from 42 min to 24 h after injection. In conclusion, manganese-enhanced MRI may open new ways for functional assessments of the habenulo-interpeduncular system in animal models with cognitive impairment.",

keywords = "Habenula, Magnetic resonance imaging, Manganese, Mice, Neural pathways",

author = "Takashi Watanabe and Jelena Radulovic and Susann Boretius and Jens Frahm and Thomas Michaelis",

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doi = "10.1016/j.mri.2005.10.034",

language = "English (US)",

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T1 - Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI

AU - Watanabe, Takashi

AU - Radulovic, Jelena

AU - Boretius, Susann

AU - Frahm, Jens

AU - Michaelis, Thomas

PY - 2006/4

Y1 - 2006/4

N2 - This magnetic resonance imaging (MRI) study describes mapping of the habenulo-interpeduncular pathway in living mice based on manganese-induced contrast. Six hours after intracerebroventricular microinjection of MnCl 2, T1-weighted 3D MRI (2.35 T) at 117 μm isotropic resolution revealed a continuous pattern of anterograde labeling from the habenula via the fasciculus retroflexus to the interpeduncular nucleus. Alternatively, the less invasive systemic administration of MnCl2 allowed for monitoring of the dynamic uptake pattern of respective neural components with even higher reproducibility across animals. Time courses covered the range from 42 min to 24 h after injection. In conclusion, manganese-enhanced MRI may open new ways for functional assessments of the habenulo-interpeduncular system in animal models with cognitive impairment.

AB - This magnetic resonance imaging (MRI) study describes mapping of the habenulo-interpeduncular pathway in living mice based on manganese-induced contrast. Six hours after intracerebroventricular microinjection of MnCl 2, T1-weighted 3D MRI (2.35 T) at 117 μm isotropic resolution revealed a continuous pattern of anterograde labeling from the habenula via the fasciculus retroflexus to the interpeduncular nucleus. Alternatively, the less invasive systemic administration of MnCl2 allowed for monitoring of the dynamic uptake pattern of respective neural components with even higher reproducibility across animals. Time courses covered the range from 42 min to 24 h after injection. In conclusion, manganese-enhanced MRI may open new ways for functional assessments of the habenulo-interpeduncular system in animal models with cognitive impairment.

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KW - Magnetic resonance imaging

KW - Manganese

KW - Mice

KW - Neural pathways

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Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI

Abstract

Keywords

ASJC Scopus subject areas

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