Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription

Rajendra Upadhya; Jae Hoon Lee; Ian M. Willis

doi:10.1016/S1097-2765(02)00787-6

Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription

Rajendra Upadhya, Jae Hoon Lee, Ian M. Willis

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182 Scopus citations

Abstract

Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S. cerevesiae that sense changes in the cellular environment and repress pol III transcription. Signaling pathways activated in response to rapamycin-induced nutrient limitation, DNA damage, and secretory pathway defects all require Maf1 in order to affect pol III transcriptional repression. In addition, Maf1 was required for repression of pol III transcription during the normal yeast growth cycle. Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.

Original language	English (US)
Pages (from-to)	1489-1494
Number of pages	6
Journal	Molecular Cell
Volume	10
Issue number	6
DOIs	https://doi.org/10.1016/S1097-2765(02)00787-6
State	Published - Dec 1 2002

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(02)00787-6

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title = "Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription",

abstract = "Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S. cerevesiae that sense changes in the cellular environment and repress pol III transcription. Signaling pathways activated in response to rapamycin-induced nutrient limitation, DNA damage, and secretory pathway defects all require Maf1 in order to affect pol III transcriptional repression. In addition, Maf1 was required for repression of pol III transcription during the normal yeast growth cycle. Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.",

author = "Rajendra Upadhya and Lee, {Jae Hoon} and Willis, {Ian M.}",

note = "Funding Information: We thank Robyn Moir for many helpful discussions and comments on the manuscript and Steve Hahn for providing us with the Rpc34 antibody. This work was supported by grants GM60631 and GM42728 from the National Institutes of Health.",

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AU - Lee, Jae Hoon

AU - Willis, Ian M.

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AB - Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S. cerevesiae that sense changes in the cellular environment and repress pol III transcription. Signaling pathways activated in response to rapamycin-induced nutrient limitation, DNA damage, and secretory pathway defects all require Maf1 in order to affect pol III transcriptional repression. In addition, Maf1 was required for repression of pol III transcription during the normal yeast growth cycle. Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.

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Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription

Abstract

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