Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1/epidermal growth factor paracrine loop

Sumanta Goswami, Erik Sahai, Jeffrey B. Wyckoff, Michael Cammer, Dianne Cox, Fiona J. Pixley, E. Richard Stanley, Jeffrey E. Segall, John S. Condeelis

Research output: Contribution to journalArticle

506 Scopus citations

Abstract

Previous studies have shown that macrophages and tumor cells are comigratory in mammary tumors and that these cell types are mutually dependent for invasion. Here we show that macrophages and tumor cells are necessary and sufficient for comigration and invasion into collagen I and that this process involves a paracrine loop. Macrophages express epidermal growth factor (EGF), which promotes the formation of elongated protrusions and cell invasion by carcinoma cells. Colony stimulating factor 1 (CSF-1) produced by carcinoma cells promotes the expression of EGF by macrophages. In addition, EGF promotes the expression of CSF-1 by carcinoma cells thereby generating a positive feedback loop. Disruption of this loop by blockade of either EGF receptor or CSF-1 receptor signaling is sufficient to inhibit both macrophage and tumor cell migration and invasion.

Original languageEnglish (US)
Pages (from-to)5278-5283
Number of pages6
JournalCancer Research
Volume65
Issue number12
DOIs
StatePublished - Jun 15 2005

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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