Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

Wendy V. Ingman, Jeff Wyckoff, Valerie Gouon-Evans, John S. Condeelis, Jeffrey W. Pollard

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Development of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1op/Csf1op). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1op/Csf1op mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds.

Original languageEnglish (US)
Pages (from-to)3222-3229
Number of pages8
JournalDevelopmental Dynamics
Volume235
Issue number12
DOIs
StatePublished - Dec 2006

Fingerprint

Human Mammary Glands
Collagen
Macrophages
Macrophage Colony-Stimulating Factor
Photons
Microscopy
Neck
Staining and Labeling
Mutation
Antibodies
Growth
Genes

Keywords

  • Collagen
  • Development
  • Macrophage
  • Mammary gland
  • Multi-photon microscope

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland. / Ingman, Wendy V.; Wyckoff, Jeff; Gouon-Evans, Valerie; Condeelis, John S.; Pollard, Jeffrey W.

In: Developmental Dynamics, Vol. 235, No. 12, 12.2006, p. 3222-3229.

Research output: Contribution to journalArticle

Ingman, Wendy V. ; Wyckoff, Jeff ; Gouon-Evans, Valerie ; Condeelis, John S. ; Pollard, Jeffrey W. / Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland. In: Developmental Dynamics. 2006 ; Vol. 235, No. 12. pp. 3222-3229.
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