Macrophages elicited with heat-killed bacillus Calmette-Guerin protect C57BL/6J mice against a syngeneic melanoma

Victoria H. Freedman, T. A. Calvelli, S. Silagi, S. C. Silverstein

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have demonstrated that a murine cytotoxic peritoneal cell can be elicited by intraperitoneal immunization with heat-killed Mycobacterium bovis, strain Bacillus Calmette-Guerin (BCG). When these cells are injected together with cells of clone B559 of B16 melanoma in a Winn-type transfer assay into syngeneic C57BL/6J mice, the tumorigenic potential of the melanoma is completely abrogated. Similarly, mice immunized intraperitoneally with dead BCG are protected against intraperitoneal challenge with a number of B16 melanoma cells sufficient to cause tumors in 100% of control mice. However, mice immunized intraperitoneally with dead BCG are not protected against tumor formation when B16 melanoma cells are injected subcutaneously. Co-injection of BCG-elicited peritoneal cells with B16 melanoma cells into nude or sublethally irradiated (650 rad) mice inhibits tumor formation in >85% of the mice, indicating that additional participation of host bone marrow- or thymus-derived leukocytes is not required to eradicate the tumor implant. The effector cell in the BCG-induced peritoneal exudate is adherent and phagocytic and is a mononuclear phagocyte. Nonadherent lymphoid cells from the same BCG-induced peritoneal exudate and from thioglycollate-broth-elicited granulocytes and macrophages neither prevent nor delay B16 tumor formation.

Original languageEnglish (US)
Pages (from-to)657-673
Number of pages17
JournalJournal of Experimental Medicine
Volume152
Issue number3
StatePublished - 1980
Externally publishedYes

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Mycobacterium bovis
Inbred C57BL Mouse
Melanoma
Hot Temperature
Macrophages
Experimental Melanomas
Exudates and Transudates
Neoplasms
Thioglycolates
Phagocytes
Granulocytes
Thymus Gland
Immunization
Leukocytes
Clone Cells
Bone Marrow
Lymphocytes
Injections

ASJC Scopus subject areas

  • Immunology

Cite this

Macrophages elicited with heat-killed bacillus Calmette-Guerin protect C57BL/6J mice against a syngeneic melanoma. / Freedman, Victoria H.; Calvelli, T. A.; Silagi, S.; Silverstein, S. C.

In: Journal of Experimental Medicine, Vol. 152, No. 3, 1980, p. 657-673.

Research output: Contribution to journalArticle

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abstract = "We have demonstrated that a murine cytotoxic peritoneal cell can be elicited by intraperitoneal immunization with heat-killed Mycobacterium bovis, strain Bacillus Calmette-Guerin (BCG). When these cells are injected together with cells of clone B559 of B16 melanoma in a Winn-type transfer assay into syngeneic C57BL/6J mice, the tumorigenic potential of the melanoma is completely abrogated. Similarly, mice immunized intraperitoneally with dead BCG are protected against intraperitoneal challenge with a number of B16 melanoma cells sufficient to cause tumors in 100{\%} of control mice. However, mice immunized intraperitoneally with dead BCG are not protected against tumor formation when B16 melanoma cells are injected subcutaneously. Co-injection of BCG-elicited peritoneal cells with B16 melanoma cells into nude or sublethally irradiated (650 rad) mice inhibits tumor formation in >85{\%} of the mice, indicating that additional participation of host bone marrow- or thymus-derived leukocytes is not required to eradicate the tumor implant. The effector cell in the BCG-induced peritoneal exudate is adherent and phagocytic and is a mononuclear phagocyte. Nonadherent lymphoid cells from the same BCG-induced peritoneal exudate and from thioglycollate-broth-elicited granulocytes and macrophages neither prevent nor delay B16 tumor formation.",
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