Low tidal volume and high positive end-expiratory pressure mechanical ventilation results in increased inflammation and ventilator-associated lung injury in normal lungs

Caron M. Hong, Da Zhong Xu, Qi Lu, Yunhui Cheng, Vadim Pisarenko, Danielle Doucet, Margaret Brown, Seena Aisner, Chunxiang Zhang, Edwin A. Deitch, Ellise Delphin

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47 Scopus citations


BACKGROUND: Protective mechanical ventilation with low tidal volume (VT) and low plateau pressure reduces mortality and decreases the length of mechanical ventilation in patients with acute respiratory distress syndrome. Mechanical ventilation that will protect normal lungs during major surgical procedures of long duration may improve postoperative outcomes. We performed an animal study comparing 3 ventilation strategies used in the operating room in normal lungs. We compared the effects on pulmonary mechanics, inflammatory mediators, and lung tissue injury. METHODS: Female pigs were randomized into 3 groups. Group H-VT/3 (n = 6) was ventilated with a VT of 15 mL/kg predicted body weight (PBW)/positive end-expiratory pressure (PEEP) of 3 cm H2O, group L-VT/3 (n = 6) with a VT of 6 mL/kg PBW/PEEP of 3 cm H2O, and group L-VT/10 (n = 6) with a VT of 6 mL/kg PBW/PEEP of 10 cm H2O, for 8 hours. Hemodynamics, airway mechanics, arterial blood gases, and inflammatory markers were monitored. Bronchoalveolar lavage (BAL) was analyzed for inflammatory markers and protein concentration. The right lower lobe was assayed for mRNA of specific cytokines. The right lower lobe and right upper lobe were evaluated histologically. RESULTS: In contrast to groups H-VT/3 and L-VT/3, group L-VT/10 exhibited a 6-fold increase in inflammatory mediators in BAL (P < 0.001). Cytokines in BAL were similar in groups H-VT/3 and L-VT/3. Group H-VT/3 had a significantly lower lung injury score than groups L-VT/3 and L-VT/10. CONCLUSION: Comparing intraoperative strategies, ventilation with high PEEP resulted in increased production of inflammatory markers. Low PEEP resulted in lower levels of inflammatory markers. High VT/low PEEP resulted in less histologic lung injury.

Original languageEnglish (US)
Pages (from-to)1652-1660
Number of pages9
JournalAnesthesia and analgesia
Issue number6
Publication statusPublished - Jun 1 2010
Externally publishedYes


ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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