Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family

Denis Odokonyero, Ayano Sakai, Yury Patskovsky, Vladimir N. Malashkevich, Alexander A. Fedorov, Jeffrey B. Bonanno, Elena V. Fedorov, Rafael Toro, Rakhi Agarwal, Chenxi Wang, Nicole D S Ozerova, Wen Shan Yew, J. Michael Sauder, Subramanyam Swaminathan, Stephen K. Burley, Steven C. Almo, Margaret E. Glasner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The rate of protein evolution is determined by a combination of selective pressure on protein function and biophysical constraints on protein folding and structure. Determining the relative contributions of these properties is an unsolved problem in molecular evolution with broad implications for protein engineering and function prediction. As a case study, we examined the structural divergence of the rapidly evolving o-succinylbenzoate synthase (OSBS) family, which catalyzes a step in menaquinone synthesis in diverse microorganisms and plants. On average, the OSBS family is much more divergent than other protein families from the same set of species, with the most divergent family members sharing <15% sequence identity. Comparing 11 representative structures revealed that loss of quaternary structure and large deletions or insertions are associated with the family's rapid evolution. Neither of these properties has been investigated in previous studies to identify factors that affect the rate of protein evolution. Intriguingly, one subfamily retained a multimeric quaternary structure and has small insertions and deletions compared with related enzymes that catalyze diverse reactions. Many proteins in this subfamily catalyze both OSBS and N-succinylamino acid racemization (NSAR). Retention of ancestral structural characteristics in the NSAR/OSBS subfamily suggests that the rate of protein evolution is not proportional to the capacity to evolve new protein functions. Instead, structural features that are conserved among proteins with diverse functions might contribute to the evolution of new functions.

Original languageEnglish (US)
Pages (from-to)8535-8540
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number23
DOIs
StatePublished - Jun 10 2014

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Proteins
Vitamin K 2
Protein Engineering
Acids
Molecular Evolution
o-succinylbenzoic acid synthase
Protein Folding
Enzymes

Keywords

  • Enolase superfamily
  • Protein structure
  • Protein structure-function relationships

ASJC Scopus subject areas

  • General

Cite this

Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family. / Odokonyero, Denis; Sakai, Ayano; Patskovsky, Yury; Malashkevich, Vladimir N.; Fedorov, Alexander A.; Bonanno, Jeffrey B.; Fedorov, Elena V.; Toro, Rafael; Agarwal, Rakhi; Wang, Chenxi; Ozerova, Nicole D S; Yew, Wen Shan; Sauder, J. Michael; Swaminathan, Subramanyam; Burley, Stephen K.; Almo, Steven C.; Glasner, Margaret E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 23, 10.06.2014, p. 8535-8540.

Research output: Contribution to journalArticle

Odokonyero, D, Sakai, A, Patskovsky, Y, Malashkevich, VN, Fedorov, AA, Bonanno, JB, Fedorov, EV, Toro, R, Agarwal, R, Wang, C, Ozerova, NDS, Yew, WS, Sauder, JM, Swaminathan, S, Burley, SK, Almo, SC & Glasner, ME 2014, 'Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 23, pp. 8535-8540. https://doi.org/10.1073/pnas.1318703111
Odokonyero, Denis ; Sakai, Ayano ; Patskovsky, Yury ; Malashkevich, Vladimir N. ; Fedorov, Alexander A. ; Bonanno, Jeffrey B. ; Fedorov, Elena V. ; Toro, Rafael ; Agarwal, Rakhi ; Wang, Chenxi ; Ozerova, Nicole D S ; Yew, Wen Shan ; Sauder, J. Michael ; Swaminathan, Subramanyam ; Burley, Stephen K. ; Almo, Steven C. ; Glasner, Margaret E. / Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 23. pp. 8535-8540.
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