Long-term culture of human endothelial cells

Portia B. Gordon, Ira I. Sussman, Victor B. Hatcher

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Human umbilical vein endothelial cells can be grown in vitro for 28 passages (CPDL 58) in Medium 199 supplemented with newborn bovine serum and a partially purified growth factor derived from bovine brain. Newborn bovine serum is superior to fetal bovine serum for the proliferation of human umbilical vein endothelial cells seeded at low density in the presence of the growth factor. The endothelial cells, which can be passaged every 7 to 10 d at a 1-to-5 split ratio, retain their morphological and biochemical characteristics. The proliferation of cells seeded at low density (103/cm2) is proportional to the concentration of the growth factor present in the medium. The growth factor, which has an isoelectric point between 5.0 and 5.5, can support cell proliferation at reduced serum concentrations; half-maximal growth is achieved in medium containing the growth factor and 3% serum. The brain endothelial cell growth factor does not stimulate DNA synthesis significantly in cultures of human skin fibroblasts.

Original languageEnglish (US)
Pages (from-to)661-671
Number of pages11
JournalIn Vitro
Volume19
Issue number9
DOIs
StatePublished - Sep 1 1983

Keywords

  • growth factor
  • human endothelial cells

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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