Long-term culture of human endothelial cells

Portia B. Gordon, Ira I. Sussman, Victor Bernard Hatcher

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Human umbilical vein endothelial cells can be grown in vitro for 28 passages (CPDL 58) in Medium 199 supplemented with newborn bovine serum and a partially purified growth factor derived from bovine brain. Newborn bovine serum is superior to fetal bovine serum for the proliferation of human umbilical vein endothelial cells seeded at low density in the presence of the growth factor. The endothelial cells, which can be passaged every 7 to 10 d at a 1-to-5 split ratio, retain their morphological and biochemical characteristics. The proliferation of cells seeded at low density (103/cm2) is proportional to the concentration of the growth factor present in the medium. The growth factor, which has an isoelectric point between 5.0 and 5.5, can support cell proliferation at reduced serum concentrations; half-maximal growth is achieved in medium containing the growth factor and 3% serum. The brain endothelial cell growth factor does not stimulate DNA synthesis significantly in cultures of human skin fibroblasts.

Original languageEnglish (US)
Pages (from-to)661-671
Number of pages11
JournalIn Vitro
Volume19
Issue number9
DOIs
StatePublished - Sep 1983

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Endothelial cells
Cell culture
Intercellular Signaling Peptides and Proteins
Endothelial Cells
Serum
Human Umbilical Vein Endothelial Cells
Endothelial Growth Factors
Cell Proliferation
Brain
Isoelectric Point
Cell proliferation
Cell growth
Fibroblasts
Skin
DNA
Growth

Keywords

  • growth factor
  • human endothelial cells

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Long-term culture of human endothelial cells. / Gordon, Portia B.; Sussman, Ira I.; Hatcher, Victor Bernard.

In: In Vitro, Vol. 19, No. 9, 09.1983, p. 661-671.

Research output: Contribution to journalArticle

Gordon, Portia B. ; Sussman, Ira I. ; Hatcher, Victor Bernard. / Long-term culture of human endothelial cells. In: In Vitro. 1983 ; Vol. 19, No. 9. pp. 661-671.
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