Locus-Specific Analysis of Human Leukocyte Antigen Class I Expression in Melanoma Cell Lines

Francesco M. Marincola, Peter Shamamian, Toni B. Simonis, Andrea Abati, Julia Hackett, Teresita O'Dea, Patricia Fetsch, John Yannelli, Nicholas P. Restifo, James J. Mule, Steven A. Rosenberg

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Surface expression of human leukocyte antigen (HLA) class I antigens on melanoma lines was evaluated by locus-specific monoclonal antibodies (mAbs) with three different techniques: Fluorescence-activated cell sorting (FACS), immunohistochemistry with cytospin preparation (ICP), and complement- mediated cytotoxicity (CMC). Eleven HLA class I-expressing cell lines developed from metastases were used. Specific expression of HLA loci was examined under routine culture conditions and after 48-h incubation in interferon-γ (IFN-γ 500 U/ ml). Loss of allelic expression was seen in one line (586-MEL): Products of genes coding for HLA-A29 and -B44, in strong linkage disequilibrium, were not detectable. HLA-A antigens were consistently detected by all methodologies and minimally affected by pretreatment with IFN- γ. HLA-B antigens were detectable in 8 of 11 lines by ICP and 3 of 11 lines by CMC. By FACS the supratypic specificity HLA-Bw6 was expressed at low levels in most lines (mean fluorescence 47.2 ± 13.4 and rose to 259.8 ± 45.9 after incubation with IFN-γ p<0.001). HLA-Cw antigen detection by CMC correlated with HLA-B (p<0.01), suggesting that down-regulation and sensitivity to IFN-γ are shared by the two loci. This low expression of the HLA-B antigens may play a role in the evasion of the host immune response and its up-regulation may be useful in allowing tumor antigen recognition.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalJournal of Immunotherapy
Volume16
Issue number1
StatePublished - 1994
Externally publishedYes

Fingerprint

HLA Antigens
Melanoma
Cell Line
Antigens
Flow Cytometry
Immunohistochemistry
N,N-dimethyl-3,3-diphenyl-1-methylallylamine
Immune Evasion
Histocompatibility Antigens Class I
Loss of Heterozygosity
Linkage Disequilibrium
Neoplasm Antigens
Interferons
Up-Regulation
Down-Regulation
Fluorescence
Monoclonal Antibodies
Neoplasm Metastasis

Keywords

  • Human leukocyte antigen class I
  • lmmunotherapy
  • Melanoma
  • ¯

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Marincola, F. M., Shamamian, P., Simonis, T. B., Abati, A., Hackett, J., O'Dea, T., ... Rosenberg, S. A. (1994). Locus-Specific Analysis of Human Leukocyte Antigen Class I Expression in Melanoma Cell Lines. Journal of Immunotherapy, 16(1), 13-23.

Locus-Specific Analysis of Human Leukocyte Antigen Class I Expression in Melanoma Cell Lines. / Marincola, Francesco M.; Shamamian, Peter; Simonis, Toni B.; Abati, Andrea; Hackett, Julia; O'Dea, Teresita; Fetsch, Patricia; Yannelli, John; Restifo, Nicholas P.; Mule, James J.; Rosenberg, Steven A.

In: Journal of Immunotherapy, Vol. 16, No. 1, 1994, p. 13-23.

Research output: Contribution to journalArticle

Marincola, FM, Shamamian, P, Simonis, TB, Abati, A, Hackett, J, O'Dea, T, Fetsch, P, Yannelli, J, Restifo, NP, Mule, JJ & Rosenberg, SA 1994, 'Locus-Specific Analysis of Human Leukocyte Antigen Class I Expression in Melanoma Cell Lines', Journal of Immunotherapy, vol. 16, no. 1, pp. 13-23.
Marincola, Francesco M. ; Shamamian, Peter ; Simonis, Toni B. ; Abati, Andrea ; Hackett, Julia ; O'Dea, Teresita ; Fetsch, Patricia ; Yannelli, John ; Restifo, Nicholas P. ; Mule, James J. ; Rosenberg, Steven A. / Locus-Specific Analysis of Human Leukocyte Antigen Class I Expression in Melanoma Cell Lines. In: Journal of Immunotherapy. 1994 ; Vol. 16, No. 1. pp. 13-23.
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