Localized control of ligand binding in hemoglobin

Effect of tertiary structure on picosecond geminate recombination

Joel M. Friedman, T. W. Scott, G. J. Fisanick, S. R. Simon, E. W. Findsen, M. R. Ondrias, V. W. Macdonald

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The picosecond geminate rebinding of molecular oxygen was monitored in a variety of different human, reptilian, and fish hemoglobins. The fast (100 to 200 picoseconds) component of the rebinding is highly sensitive to protein structure. Both proximal and distal perturbations of the heme affect this rebinding process. The rebinding yield for the fast process correlates with the frequency of the stretching motion of the iron-proximal histidine mode (νFe-His) observed in the transient Raman spectra of photodissociated ligated hemoglobins. The high-affinity R-state species exhibit the highest values for νFe-His and the highest yields for fast rebinding, whereas low affinity R-state species and T-state species exhibit lower values of νFe-His and correspondingly reduced yields for this geminate process. These findings link protein control of ligand binding with events at the heme.

Original languageEnglish (US)
Pages (from-to)187-190
Number of pages4
JournalScience
Volume229
Issue number4709
StatePublished - 1985
Externally publishedYes

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Heme
Genetic Recombination
Hemoglobins
Ligands
Histidine
Fishes
Iron
Oxygen
Proteins
link protein

ASJC Scopus subject areas

  • General

Cite this

Friedman, J. M., Scott, T. W., Fisanick, G. J., Simon, S. R., Findsen, E. W., Ondrias, M. R., & Macdonald, V. W. (1985). Localized control of ligand binding in hemoglobin: Effect of tertiary structure on picosecond geminate recombination. Science, 229(4709), 187-190.

Localized control of ligand binding in hemoglobin : Effect of tertiary structure on picosecond geminate recombination. / Friedman, Joel M.; Scott, T. W.; Fisanick, G. J.; Simon, S. R.; Findsen, E. W.; Ondrias, M. R.; Macdonald, V. W.

In: Science, Vol. 229, No. 4709, 1985, p. 187-190.

Research output: Contribution to journalArticle

Friedman, JM, Scott, TW, Fisanick, GJ, Simon, SR, Findsen, EW, Ondrias, MR & Macdonald, VW 1985, 'Localized control of ligand binding in hemoglobin: Effect of tertiary structure on picosecond geminate recombination', Science, vol. 229, no. 4709, pp. 187-190.
Friedman JM, Scott TW, Fisanick GJ, Simon SR, Findsen EW, Ondrias MR et al. Localized control of ligand binding in hemoglobin: Effect of tertiary structure on picosecond geminate recombination. Science. 1985;229(4709):187-190.
Friedman, Joel M. ; Scott, T. W. ; Fisanick, G. J. ; Simon, S. R. ; Findsen, E. W. ; Ondrias, M. R. ; Macdonald, V. W. / Localized control of ligand binding in hemoglobin : Effect of tertiary structure on picosecond geminate recombination. In: Science. 1985 ; Vol. 229, No. 4709. pp. 187-190.
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