Localization of Nopp140 within mammalian cells during interphase and mitosis

Marc Thiry, Thierry Cheutin, Françoise Lamaye, Nicolas Thelen, U. Thomas Meier, Marie Françoise O'Donohue, Dominique Ploton

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We investigated distribution of the nucleolar phosphoprotein Nopp140 within mammalian cells, using immunofluorescence confocal microscopy and immunoelectron microscopy. During interphase, three-dimensional image reconstructions of confocal sections revealed that nucleolar labelling appeared as several tiny spheres organized in necklaces. Moreover, after an immunogold labelling procedure, gold particles were detected not only over the dense fibrillar component but also over the fibrillar centres of nucleoli in untreated and actinomycin D-treated cells. Labelling was also consistently present in Cajal bodies. After pulse-chase experiments with BrUTP, colocalization was more prominent after a 10- to 15-min chase than after a 5-min chase. During mitosis, confocal analysis indicated that Nopp140 organization was lost. The protein dispersed between and around the chromosomes in prophase. From prometaphase to telophase, it was also detected in numerous cytoplasmic nucleolus-derived foci. During telophase, it reappeared in the reforming nucleoli of daughter nuclei. This strongly suggests that Nopp140 could be a component implicated in the early steps of pre-rRNA processing.

Original languageEnglish (US)
Pages (from-to)129-140
Number of pages12
JournalHistochemistry and Cell Biology
Volume132
Issue number2
DOIs
StatePublished - Aug 2009

Fingerprint

Telophase
Interphase
Mitosis
Prometaphase
Prophase
Computer-Assisted Image Processing
Immunoelectron Microscopy
Three-Dimensional Imaging
Phosphoproteins
RNA Precursors
Dactinomycin
Fluorescence Microscopy
Confocal Microscopy
Gold
Chromosomes
Proteins

Keywords

  • Confocal microscopy
  • Immunoelectron microscopy
  • Interphase
  • Mammalian cells
  • Mitosis
  • Nopp140
  • Nucleolus

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Medical Laboratory Technology
  • Molecular Biology

Cite this

Thiry, M., Cheutin, T., Lamaye, F., Thelen, N., Meier, U. T., O'Donohue, M. F., & Ploton, D. (2009). Localization of Nopp140 within mammalian cells during interphase and mitosis. Histochemistry and Cell Biology, 132(2), 129-140. https://doi.org/10.1007/s00418-009-0599-8

Localization of Nopp140 within mammalian cells during interphase and mitosis. / Thiry, Marc; Cheutin, Thierry; Lamaye, Françoise; Thelen, Nicolas; Meier, U. Thomas; O'Donohue, Marie Françoise; Ploton, Dominique.

In: Histochemistry and Cell Biology, Vol. 132, No. 2, 08.2009, p. 129-140.

Research output: Contribution to journalArticle

Thiry, M, Cheutin, T, Lamaye, F, Thelen, N, Meier, UT, O'Donohue, MF & Ploton, D 2009, 'Localization of Nopp140 within mammalian cells during interphase and mitosis', Histochemistry and Cell Biology, vol. 132, no. 2, pp. 129-140. https://doi.org/10.1007/s00418-009-0599-8
Thiry, Marc ; Cheutin, Thierry ; Lamaye, Françoise ; Thelen, Nicolas ; Meier, U. Thomas ; O'Donohue, Marie Françoise ; Ploton, Dominique. / Localization of Nopp140 within mammalian cells during interphase and mitosis. In: Histochemistry and Cell Biology. 2009 ; Vol. 132, No. 2. pp. 129-140.
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