Localization and activity of nitric oxide synthases in the gastrointestinal tract of Trypanosoma cruzi-infected mice

L. Ny; K. Persson; B. Larsson; J. Chan; L. M. Weiss; M. Wittner; H. Huang; H. B. Tanowitz

doi:10.1016/S0165-5728(99)00085-5

Localization and activity of nitric oxide synthases in the gastrointestinal tract of Trypanosoma cruzi-infected mice

L. Ny, K. Persson, B. Larsson, J. Chan, L. M. Weiss, M. Wittner, H. Huang, H. B. Tanowitz

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Chagas' disease, caused by the protozoan parasite, Trypanosoma cruzi, is associated with gastrointestinal abnormalities. Since nitric oxide (NO) has been shown to be a factor influencing intestinal function we evaluated the distributions and activities of the NO synthase (NOS) isoforms, in the gut of mice infected with T. cruzi. Ca²⁺-dependent (NOS1/NOS3) activity was decreased, whereas Ca²⁺-independent (NOS2) activity was increased in infected mice. NOS2-immunoreactivity was demonstrated in cells within the muscle layers and epithelium in infected mice and NOS1 immunoreactivity was seen in nerve structures. These data indicate that alterations in the NO- system may be important in the pathogenesis of the gastrointestinal manifestations in Chagas' disease.

Original language	English (US)
Pages (from-to)	27-35
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	99
Issue number	1
DOIs	https://doi.org/10.1016/S0165-5728(99)00085-5
State	Published - Sep 1 1999

Keywords

Chagas' disease
Enteric nervous system
Intestine
Trypanosoma cruzi

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0165-5728(99)00085-5

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title = "Localization and activity of nitric oxide synthases in the gastrointestinal tract of Trypanosoma cruzi-infected mice",

abstract = "Chagas' disease, caused by the protozoan parasite, Trypanosoma cruzi, is associated with gastrointestinal abnormalities. Since nitric oxide (NO) has been shown to be a factor influencing intestinal function we evaluated the distributions and activities of the NO synthase (NOS) isoforms, in the gut of mice infected with T. cruzi. Ca2+-dependent (NOS1/NOS3) activity was decreased, whereas Ca2+-independent (NOS2) activity was increased in infected mice. NOS2-immunoreactivity was demonstrated in cells within the muscle layers and epithelium in infected mice and NOS1 immunoreactivity was seen in nerve structures. These data indicate that alterations in the NO- system may be important in the pathogenesis of the gastrointestinal manifestations in Chagas' disease.",

keywords = "Chagas' disease, Enteric nervous system, Intestine, Trypanosoma cruzi",

author = "L. Ny and K. Persson and B. Larsson and J. Chan and Weiss, {L. M.} and M. Wittner and H. Huang and Tanowitz, {H. B.}",

note = "Funding Information: We thank Piers Emson for generous supply of NOS1 antiserum, Karl-Erik Andersson for valuable help in the planning and process of the project, and Vicki L. Braunstein, Agneta Kristensen and Christina Olsson for excellent technical support. This study was supported by the Swedish Medical Research Council grants 6837, 11205 and 12601, the Crafoord foundation, the Swedish Society for Medical Research, grants AI-12770 (HBT) and AI-41752(MW) from the United States National Institutes of Health., and by a New Investigator Award from the American Heart Association, New York City Affiliate (HH).",

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doi = "10.1016/S0165-5728(99)00085-5",

language = "English (US)",

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T1 - Localization and activity of nitric oxide synthases in the gastrointestinal tract of Trypanosoma cruzi-infected mice

AU - Ny, L.

AU - Persson, K.

AU - Larsson, B.

AU - Chan, J.

AU - Weiss, L. M.

AU - Wittner, M.

AU - Huang, H.

AU - Tanowitz, H. B.

N1 - Funding Information: We thank Piers Emson for generous supply of NOS1 antiserum, Karl-Erik Andersson for valuable help in the planning and process of the project, and Vicki L. Braunstein, Agneta Kristensen and Christina Olsson for excellent technical support. This study was supported by the Swedish Medical Research Council grants 6837, 11205 and 12601, the Crafoord foundation, the Swedish Society for Medical Research, grants AI-12770 (HBT) and AI-41752(MW) from the United States National Institutes of Health., and by a New Investigator Award from the American Heart Association, New York City Affiliate (HH).

PY - 1999/9/1

Y1 - 1999/9/1

N2 - Chagas' disease, caused by the protozoan parasite, Trypanosoma cruzi, is associated with gastrointestinal abnormalities. Since nitric oxide (NO) has been shown to be a factor influencing intestinal function we evaluated the distributions and activities of the NO synthase (NOS) isoforms, in the gut of mice infected with T. cruzi. Ca2+-dependent (NOS1/NOS3) activity was decreased, whereas Ca2+-independent (NOS2) activity was increased in infected mice. NOS2-immunoreactivity was demonstrated in cells within the muscle layers and epithelium in infected mice and NOS1 immunoreactivity was seen in nerve structures. These data indicate that alterations in the NO- system may be important in the pathogenesis of the gastrointestinal manifestations in Chagas' disease.

AB - Chagas' disease, caused by the protozoan parasite, Trypanosoma cruzi, is associated with gastrointestinal abnormalities. Since nitric oxide (NO) has been shown to be a factor influencing intestinal function we evaluated the distributions and activities of the NO synthase (NOS) isoforms, in the gut of mice infected with T. cruzi. Ca2+-dependent (NOS1/NOS3) activity was decreased, whereas Ca2+-independent (NOS2) activity was increased in infected mice. NOS2-immunoreactivity was demonstrated in cells within the muscle layers and epithelium in infected mice and NOS1 immunoreactivity was seen in nerve structures. These data indicate that alterations in the NO- system may be important in the pathogenesis of the gastrointestinal manifestations in Chagas' disease.

KW - Chagas' disease

KW - Enteric nervous system

KW - Intestine

KW - Trypanosoma cruzi

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Localization and activity of nitric oxide synthases in the gastrointestinal tract of Trypanosoma cruzi-infected mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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