Local μ-Opioid Receptor Antagonism Blunts Evoked Phasic Dopamine Release in the Nucleus Accumbens of Rats

Alexander Gómez-A, Tatiana A. Shnitko, Haley M. Barefoot, Eleanor L. Brightbill, Leslie A. Sombers, Saleem M. Nicola, Donita L. Robinson

Research output: Contribution to journalArticle

Abstract

μ-opioid receptors (MORs) in the nucleus accumbens (NAc) can regulate reward-related behaviors that are dependent on mesolimbic dopamine, but the precise mechanism of this MOR regulation is unknown. We hypothesized that MORs within the NAc core regulate dopamine release. Specifically, we infused the MOR antagonist CTAP (d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH2) into the NAc core while dopamine release was evoked by electrical stimulation of the ventral tegmental area and measured by fast-scan cyclic voltammetry. We report that CTAP dose-dependently inhibited evoked dopamine release, with full blockade achieved with the 8 μg infusion. In contrast, evoked dopamine release increased after nomifensine infusion and was unchanged after vehicle infusion. These findings demonstrate profound local control of dopamine release by MORs within the NAc core, which has implications for regulation of reward processing.

Original languageEnglish (US)
Pages (from-to)1935-1940
Number of pages6
JournalACS Chemical Neuroscience
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 17 2019

    Fingerprint

Keywords

  • accumbens
  • dopamine
  • electrochemistry
  • local infusion
  • phasic
  • μ-opioid receptor

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology

Cite this

Gómez-A, A., Shnitko, T. A., Barefoot, H. M., Brightbill, E. L., Sombers, L. A., Nicola, S. M., & Robinson, D. L. (2019). Local μ-Opioid Receptor Antagonism Blunts Evoked Phasic Dopamine Release in the Nucleus Accumbens of Rats. ACS Chemical Neuroscience, 10(4), 1935-1940. https://doi.org/10.1021/acschemneuro.8b00437