Live cell imaging of RhoGTPase biosensors in tumor cells

Jose Javier Bravo-Cordero, Yasmin Moshfegh, John S. Condeelis, Louis Hodgson

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages359-370
Number of pages12
Volume1046
ISBN (Print)9781627035378
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1046
ISSN (Print)10643745

Fingerprint

Biosensing Techniques
Actin Cytoskeleton
Cell Movement
Extracellular Matrix
Neoplasms
Membranes
Podosomes

Keywords

  • Biosensors
  • FRET
  • Invadopodia
  • RhoGTPase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bravo-Cordero, J. J., Moshfegh, Y., Condeelis, J. S., & Hodgson, L. (2013). Live cell imaging of RhoGTPase biosensors in tumor cells. In Methods in Molecular Biology (Vol. 1046, pp. 359-370). (Methods in Molecular Biology; Vol. 1046). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-538-5_22

Live cell imaging of RhoGTPase biosensors in tumor cells. / Bravo-Cordero, Jose Javier; Moshfegh, Yasmin; Condeelis, John S.; Hodgson, Louis.

Methods in Molecular Biology. Vol. 1046 Humana Press Inc., 2013. p. 359-370 (Methods in Molecular Biology; Vol. 1046).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bravo-Cordero, JJ, Moshfegh, Y, Condeelis, JS & Hodgson, L 2013, Live cell imaging of RhoGTPase biosensors in tumor cells. in Methods in Molecular Biology. vol. 1046, Methods in Molecular Biology, vol. 1046, Humana Press Inc., pp. 359-370. https://doi.org/10.1007/978-1-62703-538-5_22
Bravo-Cordero JJ, Moshfegh Y, Condeelis JS, Hodgson L. Live cell imaging of RhoGTPase biosensors in tumor cells. In Methods in Molecular Biology. Vol. 1046. Humana Press Inc. 2013. p. 359-370. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-538-5_22
Bravo-Cordero, Jose Javier ; Moshfegh, Yasmin ; Condeelis, John S. ; Hodgson, Louis. / Live cell imaging of RhoGTPase biosensors in tumor cells. Methods in Molecular Biology. Vol. 1046 Humana Press Inc., 2013. pp. 359-370 (Methods in Molecular Biology).
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