Live cell imaging of RhoGTPase biosensors in tumor cells

Jose Javier Bravo-Cordero, Yasmin Moshfegh, John Condeelis, Louis Hodgson

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.

Original languageEnglish (US)
Title of host publicationAdhesion Protein Protocols
PublisherHumana Press Inc.
Pages359-370
Number of pages12
ISBN (Print)9781627035378
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1046
ISSN (Print)1064-3745

Keywords

  • Biosensors
  • FRET
  • Invadopodia
  • RhoGTPase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Bravo-Cordero, J. J., Moshfegh, Y., Condeelis, J., & Hodgson, L. (2013). Live cell imaging of RhoGTPase biosensors in tumor cells. In Adhesion Protein Protocols (pp. 359-370). (Methods in Molecular Biology; Vol. 1046). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-538-5_22