LipC (rv0220) is an immunogenic cell surface esterase of mycobacterium tuberculosis

Guomiao Shen, Krishna Singh, Dinesh Chandra, Serveau Avesque Carole, Damien Maurin, Stéphane Canaan, Rupak Singla, Digambar Behera, Suman Laal

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Abstract

We have reported previously the identification of novel proteins of Mycobacterium tuberculosis by the immunoscreening of an expression library of M. tuberculosis genomic DNA with sera obtained from M. tuberculosis-infected rabbits at 5 weeks postinfection. In this study, we report the further characterization of one of these antigens, LipC (Rv0220). LipC is annotated as a member of the Lip family based on the presence of the consensus motif "GXSXG" characteristic of esterases. Although predicted to be a cytoplasmic enzyme, we provide evidence that LipC is a cell surface protein that is present in both the cell wall and the capsule of M. tuberculosis. Consistent with this localization, LipC elicits strong humoral immune responses in both HIV-negative (HIV -) and HIV-positive (HIV +) tuberculosis (TB) patients. The absence of anti-LipC antibodies in sera from purified protein derivative-positive (PPD +) healthy subjects confirms its expression only during active M. tuberculosis infection. Epitope mapping of LipC identified 6 immunodominant epitopes, 5 of which map to the exposed surface of the modeled LipC protein. The recombinant LipC (rLipC) protein also elicits proinflammatory cytokine and chemokine responses from macrophages and pulmonary epithelial cells. rLipC can hydrolyze short-chain esters with the carbon chain containing 2 to 10 carbon atoms. Together, these studies demonstrate that LipC is a novel cell surface-associated esterase of M. tuberculosis that is highly immunogenic and elicits both antibodies and cytokines/chemokines.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalInfection and Immunity
Volume80
Issue number1
DOIs
StatePublished - Jan 2012

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Esterases
Mycobacterium tuberculosis
HIV
Chemokines
Carbon
Cytokines
Epitope Mapping
Immunodominant Epitopes
Mycobacterium Infections
Tuberculin
Alveolar Macrophages
Humoral Immunity
Lip
Recombinant Proteins
Cell Wall
Capsules
Blood Proteins
Anti-Idiotypic Antibodies
Consensus
Healthy Volunteers

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Shen, G., Singh, K., Chandra, D., Carole, S. A., Maurin, D., Canaan, S., ... Laal, S. (2012). LipC (rv0220) is an immunogenic cell surface esterase of mycobacterium tuberculosis. Infection and Immunity, 80(1), 243-253. https://doi.org/10.1128/IAI.05541-11

LipC (rv0220) is an immunogenic cell surface esterase of mycobacterium tuberculosis. / Shen, Guomiao; Singh, Krishna; Chandra, Dinesh; Carole, Serveau Avesque; Maurin, Damien; Canaan, Stéphane; Singla, Rupak; Behera, Digambar; Laal, Suman.

In: Infection and Immunity, Vol. 80, No. 1, 01.2012, p. 243-253.

Research output: Contribution to journalArticle

Shen, G, Singh, K, Chandra, D, Carole, SA, Maurin, D, Canaan, S, Singla, R, Behera, D & Laal, S 2012, 'LipC (rv0220) is an immunogenic cell surface esterase of mycobacterium tuberculosis', Infection and Immunity, vol. 80, no. 1, pp. 243-253. https://doi.org/10.1128/IAI.05541-11
Shen, Guomiao ; Singh, Krishna ; Chandra, Dinesh ; Carole, Serveau Avesque ; Maurin, Damien ; Canaan, Stéphane ; Singla, Rupak ; Behera, Digambar ; Laal, Suman. / LipC (rv0220) is an immunogenic cell surface esterase of mycobacterium tuberculosis. In: Infection and Immunity. 2012 ; Vol. 80, No. 1. pp. 243-253.
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