TY - JOUR
T1 - Linker insertion mutagenesis of the human immunodeficiency virus reverse transcriptase expressed in bacteria
T2 - Definition of the minimal polymerase domain
AU - Prasad, V. R.
AU - Goff, S. P.
PY - 1989
Y1 - 1989
N2 - A plasmid construct expressing the p66 version of the human immunodeficiency virus reverse transcriptase as a bacterial fusion protein was subjected to in vitro mutagenesis, and the resulting variant proteins were assayed to define the locations of the two major enzymatic activities. The DNA polymerase activity was localized to the N-terminal portion of the protein; mutations altering or eliminating the C-terminal portion had little or no effect on that activity. The results suggest that, in contrast with previous reports, the p51 subunit found in virions should exhibit DNA polymerase activity. Mutations in many parts of the protein elimination RNAase H activity, suggesting that several areas are needed for proper folding and generation of that activity.
AB - A plasmid construct expressing the p66 version of the human immunodeficiency virus reverse transcriptase as a bacterial fusion protein was subjected to in vitro mutagenesis, and the resulting variant proteins were assayed to define the locations of the two major enzymatic activities. The DNA polymerase activity was localized to the N-terminal portion of the protein; mutations altering or eliminating the C-terminal portion had little or no effect on that activity. The results suggest that, in contrast with previous reports, the p51 subunit found in virions should exhibit DNA polymerase activity. Mutations in many parts of the protein elimination RNAase H activity, suggesting that several areas are needed for proper folding and generation of that activity.
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U2 - 10.1073/pnas.86.9.3104
DO - 10.1073/pnas.86.9.3104
M3 - Article
C2 - 2470090
AN - SCOPUS:0024550382
SN - 0027-8424
VL - 86
SP - 3104
EP - 3108
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -