TY - JOUR
T1 - Limits of immunohistochemistry in the diagnosis of epithelial downgrowth
AU - Bergwerk, K. L.
AU - Rosenbaum, P. S.
AU - Tanaka, K. E.
AU - Udeil, I. J.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose: The immunohistochemical staining characteristics of corneal endothelium was studied to clarify the usefulness of these stains in the pathologic diagnosis of epithelial downgrowth. Methods: Tissue sections of formalin-fixed, paraffin-embedded corneal specimens (keratoconus, pseudophakic bullous keratopathy, epithelial downgrowth, failed corneal grafts with retrocorneal fibrous membranes, and postmortem control corneas) were immunostained with a panel of corneal epithelial and endothelial markers (EMA, KAE 1/3, CAM5.2, Factor VIII, CD-34, CD-31) and with Vimentin using the Strept-Avidin technique (Dako). Results: Corneal endothelium is immunonegative for endothelial cell markers (CD-34,CD-31, and Factor VIII) and displays immunopositivity for epithelial markers (KAE 1/3, CAM 5.2). Both retrocorneal fibrous membranes and epithelial downgrowth were immunopositive for KAE1/3, CAMS.2, and Vimentin, with epithelial downgrowth additionally positive for EMA. Conclusion: Immunohistochemistry is of limited usefulness in the pathologic differentiation of corneal endothelium from epithelial downgrowth and from retrocorneal fibrous membrane formation.
AB - Purpose: The immunohistochemical staining characteristics of corneal endothelium was studied to clarify the usefulness of these stains in the pathologic diagnosis of epithelial downgrowth. Methods: Tissue sections of formalin-fixed, paraffin-embedded corneal specimens (keratoconus, pseudophakic bullous keratopathy, epithelial downgrowth, failed corneal grafts with retrocorneal fibrous membranes, and postmortem control corneas) were immunostained with a panel of corneal epithelial and endothelial markers (EMA, KAE 1/3, CAM5.2, Factor VIII, CD-34, CD-31) and with Vimentin using the Strept-Avidin technique (Dako). Results: Corneal endothelium is immunonegative for endothelial cell markers (CD-34,CD-31, and Factor VIII) and displays immunopositivity for epithelial markers (KAE 1/3, CAM 5.2). Both retrocorneal fibrous membranes and epithelial downgrowth were immunopositive for KAE1/3, CAMS.2, and Vimentin, with epithelial downgrowth additionally positive for EMA. Conclusion: Immunohistochemistry is of limited usefulness in the pathologic differentiation of corneal endothelium from epithelial downgrowth and from retrocorneal fibrous membrane formation.
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M3 - Article
AN - SCOPUS:33750164015
SN - 0146-0404
VL - 37
SP - S83
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 3
ER -