Limits of immunohistochemistry in the diagnosis of epithelial downgrowth

K. L. Bergwerk, P. S. Rosenbaum, Kathryn E. Tanaka, I. J. Udeil

Research output: Contribution to journalArticle

Abstract

Purpose: The immunohistochemical staining characteristics of corneal endothelium was studied to clarify the usefulness of these stains in the pathologic diagnosis of epithelial downgrowth. Methods: Tissue sections of formalin-fixed, paraffin-embedded corneal specimens (keratoconus, pseudophakic bullous keratopathy, epithelial downgrowth, failed corneal grafts with retrocorneal fibrous membranes, and postmortem control corneas) were immunostained with a panel of corneal epithelial and endothelial markers (EMA, KAE 1/3, CAM5.2, Factor VIII, CD-34, CD-31) and with Vimentin using the Strept-Avidin technique (Dako). Results: Corneal endothelium is immunonegative for endothelial cell markers (CD-34,CD-31, and Factor VIII) and displays immunopositivity for epithelial markers (KAE 1/3, CAM 5.2). Both retrocorneal fibrous membranes and epithelial downgrowth were immunopositive for KAE1/3, CAMS.2, and Vimentin, with epithelial downgrowth additionally positive for EMA. Conclusion: Immunohistochemistry is of limited usefulness in the pathologic differentiation of corneal endothelium from epithelial downgrowth and from retrocorneal fibrous membrane formation.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

Fingerprint

Corneal Endothelium
Immunohistochemistry
Factor VIII
Vimentin
Membranes
Keratoconus
Avidin
Paraffin
Cornea
Formaldehyde
Coloring Agents
Endothelial Cells
Staining and Labeling
Transplants

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Bergwerk, K. L., Rosenbaum, P. S., Tanaka, K. E., & Udeil, I. J. (1996). Limits of immunohistochemistry in the diagnosis of epithelial downgrowth. Investigative Ophthalmology and Visual Science, 37(3).

Limits of immunohistochemistry in the diagnosis of epithelial downgrowth. / Bergwerk, K. L.; Rosenbaum, P. S.; Tanaka, Kathryn E.; Udeil, I. J.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Bergwerk, KL, Rosenbaum, PS, Tanaka, KE & Udeil, IJ 1996, 'Limits of immunohistochemistry in the diagnosis of epithelial downgrowth', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Bergwerk, K. L. ; Rosenbaum, P. S. ; Tanaka, Kathryn E. ; Udeil, I. J. / Limits of immunohistochemistry in the diagnosis of epithelial downgrowth. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
@article{df3c4945221a4402803c748ba4d53435,
title = "Limits of immunohistochemistry in the diagnosis of epithelial downgrowth",
abstract = "Purpose: The immunohistochemical staining characteristics of corneal endothelium was studied to clarify the usefulness of these stains in the pathologic diagnosis of epithelial downgrowth. Methods: Tissue sections of formalin-fixed, paraffin-embedded corneal specimens (keratoconus, pseudophakic bullous keratopathy, epithelial downgrowth, failed corneal grafts with retrocorneal fibrous membranes, and postmortem control corneas) were immunostained with a panel of corneal epithelial and endothelial markers (EMA, KAE 1/3, CAM5.2, Factor VIII, CD-34, CD-31) and with Vimentin using the Strept-Avidin technique (Dako). Results: Corneal endothelium is immunonegative for endothelial cell markers (CD-34,CD-31, and Factor VIII) and displays immunopositivity for epithelial markers (KAE 1/3, CAM 5.2). Both retrocorneal fibrous membranes and epithelial downgrowth were immunopositive for KAE1/3, CAMS.2, and Vimentin, with epithelial downgrowth additionally positive for EMA. Conclusion: Immunohistochemistry is of limited usefulness in the pathologic differentiation of corneal endothelium from epithelial downgrowth and from retrocorneal fibrous membrane formation.",
author = "Bergwerk, {K. L.} and Rosenbaum, {P. S.} and Tanaka, {Kathryn E.} and Udeil, {I. J.}",
year = "1996",
month = "2",
day = "15",
language = "English (US)",
volume = "37",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Limits of immunohistochemistry in the diagnosis of epithelial downgrowth

AU - Bergwerk, K. L.

AU - Rosenbaum, P. S.

AU - Tanaka, Kathryn E.

AU - Udeil, I. J.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose: The immunohistochemical staining characteristics of corneal endothelium was studied to clarify the usefulness of these stains in the pathologic diagnosis of epithelial downgrowth. Methods: Tissue sections of formalin-fixed, paraffin-embedded corneal specimens (keratoconus, pseudophakic bullous keratopathy, epithelial downgrowth, failed corneal grafts with retrocorneal fibrous membranes, and postmortem control corneas) were immunostained with a panel of corneal epithelial and endothelial markers (EMA, KAE 1/3, CAM5.2, Factor VIII, CD-34, CD-31) and with Vimentin using the Strept-Avidin technique (Dako). Results: Corneal endothelium is immunonegative for endothelial cell markers (CD-34,CD-31, and Factor VIII) and displays immunopositivity for epithelial markers (KAE 1/3, CAM 5.2). Both retrocorneal fibrous membranes and epithelial downgrowth were immunopositive for KAE1/3, CAMS.2, and Vimentin, with epithelial downgrowth additionally positive for EMA. Conclusion: Immunohistochemistry is of limited usefulness in the pathologic differentiation of corneal endothelium from epithelial downgrowth and from retrocorneal fibrous membrane formation.

AB - Purpose: The immunohistochemical staining characteristics of corneal endothelium was studied to clarify the usefulness of these stains in the pathologic diagnosis of epithelial downgrowth. Methods: Tissue sections of formalin-fixed, paraffin-embedded corneal specimens (keratoconus, pseudophakic bullous keratopathy, epithelial downgrowth, failed corneal grafts with retrocorneal fibrous membranes, and postmortem control corneas) were immunostained with a panel of corneal epithelial and endothelial markers (EMA, KAE 1/3, CAM5.2, Factor VIII, CD-34, CD-31) and with Vimentin using the Strept-Avidin technique (Dako). Results: Corneal endothelium is immunonegative for endothelial cell markers (CD-34,CD-31, and Factor VIII) and displays immunopositivity for epithelial markers (KAE 1/3, CAM 5.2). Both retrocorneal fibrous membranes and epithelial downgrowth were immunopositive for KAE1/3, CAMS.2, and Vimentin, with epithelial downgrowth additionally positive for EMA. Conclusion: Immunohistochemistry is of limited usefulness in the pathologic differentiation of corneal endothelium from epithelial downgrowth and from retrocorneal fibrous membrane formation.

UR - http://www.scopus.com/inward/record.url?scp=33750164015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750164015&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750164015

VL - 37

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -