LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells

Chaker N. Adra, Shaochun Zhu, Jone Long Ko, Jean Claude Guillemot, Ana Maria Cuervo, Hirofumi Kobayashi, Tadashi Horiuchi, Jean Michel Lelias, Janet D. Rowley, Bing Lim

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

While a large body of knowledge about cell membrane proteins exists, much less is known about the repertoire and function of integral membrane proteins of intracellular organelles. In looking for novel classes of genes that are functionally important to hematopoietic cells, we have cloned the cDNA for a gene preferentially expressed in adult hematopoietic tissues. During embryonic development the gene is expressed in both hematopoietic and nonhematopoietic tissues. In cell lines the gene is expressed specifically in hematopoietic lineages, whereas in normal adult tissues the mRNA is preferentially detected at high levels in lymphoid and myeloid tissues. The predicted protein is a pentaspanner with no homology to known genes and conserved across evolution. Immunocytological and cell fractionation studies with a specific antibody revealed a protein localizing in lysosomes. The gene, provisionally named LAPTM5, maps to chromosome 1p34. The expression pattern of the gene together with preliminary evidence that the protein interacts with ubiquitin indicates that the protein may have a special functional role during embryogenesis and in adult hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)328-337
Number of pages10
JournalGenomics
Volume35
Issue number2
DOIs
StatePublished - Jul 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Adra, C. N., Zhu, S., Ko, J. L., Guillemot, J. C., Cuervo, A. M., Kobayashi, H., Horiuchi, T., Lelias, J. M., Rowley, J. D., & Lim, B. (1996). LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. Genomics, 35(2), 328-337. https://doi.org/10.1006/geno.1996.0364