LAPTM5

A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells

Chaker N. Adra, Shaochun Zhu, Jone Long Ko, Jean Claude Guillemot, Ana Maria Cuervo, Hirofumi Kobayashi, Tadashi Horiuchi, Jean Michel Lelias, Janet D. Rowley, Bing Lim

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

While a large body of knowledge about cell membrane proteins exists, much less is known about the repertoire and function of integral membrane proteins of intracellular organelles. In looking for novel classes of genes that are functionally important to hematopoietic cells, we have cloned the cDNA for a gene preferentially expressed in adult hematopoietic tissues. During embryonic development the gene is expressed in both hematopoietic and nonhematopoietic tissues. In cell lines the gene is expressed specifically in hematopoietic lineages, whereas in normal adult tissues the mRNA is preferentially detected at high levels in lymphoid and myeloid tissues. The predicted protein is a pentaspanner with no homology to known genes and conserved across evolution. Immunocytological and cell fractionation studies with a specific antibody revealed a protein localizing in lysosomes. The gene, provisionally named LAPTM5, maps to chromosome 1p34. The expression pattern of the gene together with preliminary evidence that the protein interacts with ubiquitin indicates that the protein may have a special functional role during embryogenesis and in adult hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)328-337
Number of pages10
JournalGenomics
Volume35
Issue number2
DOIs
StatePublished - Jul 15 1996
Externally publishedYes

Fingerprint

Lysosome-Associated Membrane Glycoproteins
Genes
Embryonic Development
Membrane Proteins
Proteins
Hydroxyethyl Starch Derivatives
Cell Fractionation
Lymphoid Tissue
Ubiquitin
Lysosomes
Organelles
Complementary DNA
Chromosomes
Gene Expression
Cell Line
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Genetics

Cite this

LAPTM5 : A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. / Adra, Chaker N.; Zhu, Shaochun; Ko, Jone Long; Guillemot, Jean Claude; Cuervo, Ana Maria; Kobayashi, Hirofumi; Horiuchi, Tadashi; Lelias, Jean Michel; Rowley, Janet D.; Lim, Bing.

In: Genomics, Vol. 35, No. 2, 15.07.1996, p. 328-337.

Research output: Contribution to journalArticle

Adra, CN, Zhu, S, Ko, JL, Guillemot, JC, Cuervo, AM, Kobayashi, H, Horiuchi, T, Lelias, JM, Rowley, JD & Lim, B 1996, 'LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells', Genomics, vol. 35, no. 2, pp. 328-337. https://doi.org/10.1006/geno.1996.0364
Adra, Chaker N. ; Zhu, Shaochun ; Ko, Jone Long ; Guillemot, Jean Claude ; Cuervo, Ana Maria ; Kobayashi, Hirofumi ; Horiuchi, Tadashi ; Lelias, Jean Michel ; Rowley, Janet D. ; Lim, Bing. / LAPTM5 : A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells. In: Genomics. 1996 ; Vol. 35, No. 2. pp. 328-337.
@article{c169f28849134ff3aaae1585c046aabc,
title = "LAPTM5: A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells",
abstract = "While a large body of knowledge about cell membrane proteins exists, much less is known about the repertoire and function of integral membrane proteins of intracellular organelles. In looking for novel classes of genes that are functionally important to hematopoietic cells, we have cloned the cDNA for a gene preferentially expressed in adult hematopoietic tissues. During embryonic development the gene is expressed in both hematopoietic and nonhematopoietic tissues. In cell lines the gene is expressed specifically in hematopoietic lineages, whereas in normal adult tissues the mRNA is preferentially detected at high levels in lymphoid and myeloid tissues. The predicted protein is a pentaspanner with no homology to known genes and conserved across evolution. Immunocytological and cell fractionation studies with a specific antibody revealed a protein localizing in lysosomes. The gene, provisionally named LAPTM5, maps to chromosome 1p34. The expression pattern of the gene together with preliminary evidence that the protein interacts with ubiquitin indicates that the protein may have a special functional role during embryogenesis and in adult hematopoietic cells.",
author = "Adra, {Chaker N.} and Shaochun Zhu and Ko, {Jone Long} and Guillemot, {Jean Claude} and Cuervo, {Ana Maria} and Hirofumi Kobayashi and Tadashi Horiuchi and Lelias, {Jean Michel} and Rowley, {Janet D.} and Bing Lim",
year = "1996",
month = "7",
day = "15",
doi = "10.1006/geno.1996.0364",
language = "English (US)",
volume = "35",
pages = "328--337",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - LAPTM5

T2 - A novel lysosomal-associated multispanning membrane protein preferentially expressed in hematopoietic cells

AU - Adra, Chaker N.

AU - Zhu, Shaochun

AU - Ko, Jone Long

AU - Guillemot, Jean Claude

AU - Cuervo, Ana Maria

AU - Kobayashi, Hirofumi

AU - Horiuchi, Tadashi

AU - Lelias, Jean Michel

AU - Rowley, Janet D.

AU - Lim, Bing

PY - 1996/7/15

Y1 - 1996/7/15

N2 - While a large body of knowledge about cell membrane proteins exists, much less is known about the repertoire and function of integral membrane proteins of intracellular organelles. In looking for novel classes of genes that are functionally important to hematopoietic cells, we have cloned the cDNA for a gene preferentially expressed in adult hematopoietic tissues. During embryonic development the gene is expressed in both hematopoietic and nonhematopoietic tissues. In cell lines the gene is expressed specifically in hematopoietic lineages, whereas in normal adult tissues the mRNA is preferentially detected at high levels in lymphoid and myeloid tissues. The predicted protein is a pentaspanner with no homology to known genes and conserved across evolution. Immunocytological and cell fractionation studies with a specific antibody revealed a protein localizing in lysosomes. The gene, provisionally named LAPTM5, maps to chromosome 1p34. The expression pattern of the gene together with preliminary evidence that the protein interacts with ubiquitin indicates that the protein may have a special functional role during embryogenesis and in adult hematopoietic cells.

AB - While a large body of knowledge about cell membrane proteins exists, much less is known about the repertoire and function of integral membrane proteins of intracellular organelles. In looking for novel classes of genes that are functionally important to hematopoietic cells, we have cloned the cDNA for a gene preferentially expressed in adult hematopoietic tissues. During embryonic development the gene is expressed in both hematopoietic and nonhematopoietic tissues. In cell lines the gene is expressed specifically in hematopoietic lineages, whereas in normal adult tissues the mRNA is preferentially detected at high levels in lymphoid and myeloid tissues. The predicted protein is a pentaspanner with no homology to known genes and conserved across evolution. Immunocytological and cell fractionation studies with a specific antibody revealed a protein localizing in lysosomes. The gene, provisionally named LAPTM5, maps to chromosome 1p34. The expression pattern of the gene together with preliminary evidence that the protein interacts with ubiquitin indicates that the protein may have a special functional role during embryogenesis and in adult hematopoietic cells.

UR - http://www.scopus.com/inward/record.url?scp=0030586233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030586233&partnerID=8YFLogxK

U2 - 10.1006/geno.1996.0364

DO - 10.1006/geno.1996.0364

M3 - Article

VL - 35

SP - 328

EP - 337

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -