TY - JOUR
T1 - Lack of functionally active Melan-A26-35-specific T cells in the blood of HLA-A2+ vitiligo patients
AU - Adams, Sylvia
AU - Lowes, Michelle A.
AU - O'Neill, David W.
AU - Schachterle, Stephen
AU - Romero, Pedro
AU - Bhardwaj, Nina
N1 - Funding Information:
S.A. was supported by an American Society of Clinical Oncology Young Investigator Award and Career Development Award and NIH 5P30CA016087, M.A.L. by the Clinical Scholars Program at Rockefeller University, D.W.O. by NIH 5P30CA016087, P.R. by European Community FP6 grant, Cancerimmunotherapy, and N.B. by NIH 5R01AIO61684, Doris Duke Charitable Foundation 20010852, the Emerald Foundation, and Burroughs Wellcome Fund Clinical Scientist Award.
PY - 2008/8
Y1 - 2008/8
N2 - Vitiligo, a skin disorder characterized by the spontaneous destruction of melanocytes, is believed to be of autoimmune origin. We investigated the presence and functionality of CD8+ T-cells specific for the melanocyte-associated antigens Melan-A, gp100, tyrosinase, and TRP-2 in the blood of HLA-A2+ vitiligo patients. We enumerated antigen-specific CD8+ T cells by major histocompatibility complex multimer staining directly ex vivo, as well as after 9 days of in vitro stimulation and assessed IFN-γ secretion by enzyme-linked immunospot (Elispot) assay. Tyrosinase-, gp100-, or TRP-2-specific CD8+ T cells could not be identified in the peripheral blood of individuals with vitiligo. Although Melan-A-specific T cells were detectable at levels comparable to Flu-MP-specific T cells by multimer staining, these lymphocytes did not express the skin-homing receptor cutaneous lymphocyte antigen, were phenotypically naïve (CD45RA +), and were unresponsive in the IFN-γ Elispot assay, suggesting that they are unlikely to be involved in the etiopathogenesis of vitiligo.
AB - Vitiligo, a skin disorder characterized by the spontaneous destruction of melanocytes, is believed to be of autoimmune origin. We investigated the presence and functionality of CD8+ T-cells specific for the melanocyte-associated antigens Melan-A, gp100, tyrosinase, and TRP-2 in the blood of HLA-A2+ vitiligo patients. We enumerated antigen-specific CD8+ T cells by major histocompatibility complex multimer staining directly ex vivo, as well as after 9 days of in vitro stimulation and assessed IFN-γ secretion by enzyme-linked immunospot (Elispot) assay. Tyrosinase-, gp100-, or TRP-2-specific CD8+ T cells could not be identified in the peripheral blood of individuals with vitiligo. Although Melan-A-specific T cells were detectable at levels comparable to Flu-MP-specific T cells by multimer staining, these lymphocytes did not express the skin-homing receptor cutaneous lymphocyte antigen, were phenotypically naïve (CD45RA +), and were unresponsive in the IFN-γ Elispot assay, suggesting that they are unlikely to be involved in the etiopathogenesis of vitiligo.
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U2 - 10.1038/jid.2008.31
DO - 10.1038/jid.2008.31
M3 - Article
C2 - 18337837
AN - SCOPUS:47349089765
SN - 0022-202X
VL - 128
SP - 1977
EP - 1980
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 8
ER -