Label-free photoacoustic nanoscopy

Amos Danielli, Konstantin Maslov, Alejandro Garcia-Uribe, Amy M. Winkler, Chiye Li, Lidai Wang, Yun Chen, Gerald W. Dorn, Lihong V. Wang

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Super-resolution microscopy techniques-capable of overcoming the diffraction limit of light-have opened new opportunities to explore subcellular structures and dynamics not resolvable in conventional far-field microscopy. However, relying on staining with exogenous fluorescent markers, these techniques can sometimes introduce undesired artifacts to the image, mainly due to large tagging agent sizes and insufficient or variable labeling densities. By contrast, the use of endogenous pigments allows imaging of the intrinsic structures of biological samples with unaltered molecular constituents. Here, we report label-free photoacoustic (PA) nanoscopy, which is exquisitely sensitive to optical absorption, with an 88 nm resolution. At each scanning position, multiple PA signals are successively excited with increasing laser pulse energy. Because of optical saturation or nonlinear thermal expansion, the PA amplitude depends on the nonlinear incident optical fluence. The high-order dependence, quantified by polynomial fitting, provides super-resolution imaging with optical sectioning. PA nanoscopy iscapable of super-resolution imaging of either fluorescent or nonfluorescent molecules.

Original languageEnglish (US)
Article number086006
JournalJournal of Biomedical Optics
Volume19
Issue number8
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Photoacoustic effect
Labels
Imaging techniques
marking
Microscopic examination
microscopy
staining
pigments
Pigments
markers
Light absorption
Labeling
Thermal expansion
far fields
artifacts
thermal expansion
Laser pulses
fluence
polynomials
optical absorption

Keywords

  • label-free
  • microscopy
  • mitochondria
  • nanoscopy
  • photoacoustics
  • super-resolution

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Danielli, A., Maslov, K., Garcia-Uribe, A., Winkler, A. M., Li, C., Wang, L., ... Wang, L. V. (2014). Label-free photoacoustic nanoscopy. Journal of Biomedical Optics, 19(8), [086006]. https://doi.org/10.1117/1.JBO.19.8.086006

Label-free photoacoustic nanoscopy. / Danielli, Amos; Maslov, Konstantin; Garcia-Uribe, Alejandro; Winkler, Amy M.; Li, Chiye; Wang, Lidai; Chen, Yun; Dorn, Gerald W.; Wang, Lihong V.

In: Journal of Biomedical Optics, Vol. 19, No. 8, 086006, 2014.

Research output: Contribution to journalArticle

Danielli, A, Maslov, K, Garcia-Uribe, A, Winkler, AM, Li, C, Wang, L, Chen, Y, Dorn, GW & Wang, LV 2014, 'Label-free photoacoustic nanoscopy', Journal of Biomedical Optics, vol. 19, no. 8, 086006. https://doi.org/10.1117/1.JBO.19.8.086006
Danielli A, Maslov K, Garcia-Uribe A, Winkler AM, Li C, Wang L et al. Label-free photoacoustic nanoscopy. Journal of Biomedical Optics. 2014;19(8). 086006. https://doi.org/10.1117/1.JBO.19.8.086006
Danielli, Amos ; Maslov, Konstantin ; Garcia-Uribe, Alejandro ; Winkler, Amy M. ; Li, Chiye ; Wang, Lidai ; Chen, Yun ; Dorn, Gerald W. ; Wang, Lihong V. / Label-free photoacoustic nanoscopy. In: Journal of Biomedical Optics. 2014 ; Vol. 19, No. 8.
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