L5 luciferase reporter mycobacteriophages: a sensitive tool for the detection and assay of live mycobacteria

Gary J. Sarkis, William R. Jacobs, Graham F. Hatfulll

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Recombinant bacteriophages provide efficient delivery systems for introducing reporter genes into specific bacterial hosts. We have constructed mycobacteriophage L5 recombinants carrying the firefly luciferase gene inserted into the tRNA region of the phage genome. Infection of Mycobacterium smegmatis by these phages results in expression of the luciferase gene and light emission. Fortuitously, the luciferase gene is expressed continuously in lysogens surviving infection. Synthesis of luciferase from a mycobacterial promoter created by cloning enables the detection of extremely small numbers of M. smegmatis cells. These reporter phages can be used to discriminate between drug‐sensitive and drug‐resistant strains of M. smegmatis, and may provide tools for the rapid identification and classification of antimycobacterial agents.

Original languageEnglish (US)
Pages (from-to)1055-1067
Number of pages13
JournalMolecular Microbiology
Volume15
Issue number6
DOIs
StatePublished - Mar 1995

Fingerprint

Mycobacteriophages
Mycobacterium
Luciferases
Mycobacterium smegmatis
Bacteriophages
Firefly Luciferases
Transfer RNA
Infection
Reporter Genes
Genes
Organism Cloning
Genome
Anti-Bacterial Agents
Gene Expression
Light

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

L5 luciferase reporter mycobacteriophages : a sensitive tool for the detection and assay of live mycobacteria. / Sarkis, Gary J.; Jacobs, William R.; Hatfulll, Graham F.

In: Molecular Microbiology, Vol. 15, No. 6, 03.1995, p. 1055-1067.

Research output: Contribution to journalArticle

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