L-theanine inhibits nicotine-induced dependence via regulation of the nicotine acetylcholine receptor-dopamine reward pathway

Xiao Jing Di, Jingqi Yan, Yan Zhao, Yan Zhong Chang, Bao Lu Zhao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, the inhibitory effect of L-theanine, an amino acid derivative of tea, on the rewarding effects of nicotine and its underlying mechanisms of action were studied. We found that L-theanine inhibited the rewarding effects of nicotine in a conditioned place preference (CPP) model of the mouse and reduced the excitatory status induced by nicotine in SH-SY5Y cells to the same extent as the nicotine receptor inhibitor dihydro-beta-erythroidine (DHβE). Further studies using high performance liquid chromatography, western blotting and immunofluorescence staining analyses showed that L-theanine significantly inhibited nicotine-induced tyrosine hydroxylase (TH) expression and dopamine production in the midbrain of mice. L-theanine treatment also reduced the upregulation of the α4, β2 and α7 nicotine acetylcholine receptor (nAChR) subunits induced by nicotine in mouse brain regions that related to the dopamine reward pathway, thus decreasing the number of cells that could react to nicotine. In addition, L-theanine treatment inhibited nicotine-induced c-Fos expression in the reward circuit related areas of the mouse brain. Knockdown of c-Fos by siRNA inhibited the excitatory status of cells but not the upregulation of TH induced by nicotine in SH-SY5Y cells. Overall, the present study showed that L-theanine reduced the nicotine-induced reward effects via inhibition of the nAChR-dopamine reward pathway. These results may offer new therapeutic strategies for treatment of tobacco addiction.

Original languageEnglish (US)
Pages (from-to)1064-1074
Number of pages11
JournalScience China Life Sciences
Volume55
Issue number12
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

theanine
Tobacco Use Disorder
cholinergic receptors
nicotine
Cholinergic Receptors
dopamine
Nicotine
Reward
Dopamine
brain
tyrosine 3-monooxygenase
tea
tobacco
liquid chromatography
inhibitor
amino acid
Tyrosine 3-Monooxygenase
effect
regulation
Brain

Keywords

  • conditioned place preference (CPP)
  • dopamine
  • L-theanine
  • nicotine acetylcholine receptor (nAChR)
  • nicotine addiction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

L-theanine inhibits nicotine-induced dependence via regulation of the nicotine acetylcholine receptor-dopamine reward pathway. / Di, Xiao Jing; Yan, Jingqi; Zhao, Yan; Chang, Yan Zhong; Zhao, Bao Lu.

In: Science China Life Sciences, Vol. 55, No. 12, 01.12.2012, p. 1064-1074.

Research output: Contribution to journalArticle

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