TY - JOUR
T1 - Krüppel-like factor 7 is required for olfactory bulb dopaminergic neuron development
AU - Caiazzo, Massimiliano
AU - Colucci-D'Amato, Luca
AU - Volpicelli, Floriana
AU - Speranza, Luisa
AU - Petrone, Ciro
AU - Pastore, Lucio
AU - Stifani, Stefano
AU - Ramirez, Francesco
AU - Bellenchi, Gian Carlo
AU - di Porzio, Umberto
N1 - Funding Information:
This work was supported by MIUR PRIN 2007F7AJYJ_004 and 2007STRNHK_002 , by the Italian Ministry of Health Young-investigator project “under-40 call 2007”, by MERIT Program RBNE08LN4P_002 , and by FIRB International 2006 RBIN062YH4 .
Funding Information:
S.S. was supported by funds from the Canadian Institutes of Health Research ( MOP-13957 ) and by a Chercheur National Award from the Fonds de la Recherche en Sante du Quebec.
PY - 2011/2/15
Y1 - 2011/2/15
N2 - Krüppel-like factor 7 (KLF7) belongs to the large family of KLF transcription factors, which comprises at least 17 members. Within this family, KLF7 is unique since its expression is strictly restricted within the nervous system during development. We have previously shown that KLF7 is required for neuronal morphogenesis and axon guidance in selected regions of the nervous system, including hippocampus, olfactory bulbs and cortex, as well as in neuronal cell cultures. In the present work, we have furthered our analysis of the role of KLF7 in central nervous system development. By gene expression analysis during brain embryogenesis, we found significant alterations in dopaminergic neurons in Klf7 null mice. In particular, the tyrosine hydroxylase (TH) and dopamine transporter (Dat) transcripts are strongly decreased in the olfactory bulbs and ventral midbrain at birth, compared to wild-type littermates. Interestingly, Klf7-mutant mice show a dramatic reduction of TH-positive neurons in the olfactory bulbs, but no change in GABAergic or midbrain dopaminergic neurons. These observations raise the possibility that a lack of a KLF family member affects dopaminergic neuron development.
AB - Krüppel-like factor 7 (KLF7) belongs to the large family of KLF transcription factors, which comprises at least 17 members. Within this family, KLF7 is unique since its expression is strictly restricted within the nervous system during development. We have previously shown that KLF7 is required for neuronal morphogenesis and axon guidance in selected regions of the nervous system, including hippocampus, olfactory bulbs and cortex, as well as in neuronal cell cultures. In the present work, we have furthered our analysis of the role of KLF7 in central nervous system development. By gene expression analysis during brain embryogenesis, we found significant alterations in dopaminergic neurons in Klf7 null mice. In particular, the tyrosine hydroxylase (TH) and dopamine transporter (Dat) transcripts are strongly decreased in the olfactory bulbs and ventral midbrain at birth, compared to wild-type littermates. Interestingly, Klf7-mutant mice show a dramatic reduction of TH-positive neurons in the olfactory bulbs, but no change in GABAergic or midbrain dopaminergic neurons. These observations raise the possibility that a lack of a KLF family member affects dopaminergic neuron development.
KW - Adult neurogenesis
KW - Dopamine transporter
KW - Neuronal differentiation
KW - Olfactory bulbs
KW - Tyrosine hydroxylase
KW - Ventral midbrain
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U2 - 10.1016/j.yexcr.2010.11.006
DO - 10.1016/j.yexcr.2010.11.006
M3 - Article
AN - SCOPUS:78651453513
SN - 0014-4827
VL - 317
SP - 464
EP - 473
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 4
ER -