TY - JOUR
T1 - KpnI families of long, interspersed repetitive DNAs in human and other primate genomes
AU - Shafit-Zagardo, Bridget
AU - Maio, Joseph J.
AU - Brown, Fred L.
N1 - Funding Information:
ACKNOWLEDGMENTS We thank Drs. T. Maniatis and A. Bank for the gifts of the human genome libraries, Drs. P. Deininger and C. Schmid for the Blur 8 clone containing the Alu family sequence, Drs. L. Eckhardt and L. Leinwand for their help and advice and Ms. D. Dechiaro who prepared the manuscript. These studies were supported by National Institutes of Health Grants CA16790 (National Cancer Institute) and GM19100 (Institute of General Medical Sciences).
PY - 1982/5/25
Y1 - 1982/5/25
N2 - KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately cloned in the KpnI site of a plasmid pBK5, specially constructed for this purpose. The KpnI clones did not cross-hybridize with cloned, primate alphoid sequences, suggesting that the KpnI families represent sequences separate and distinct from the alphoid DNAs. Secondary restriction analyses of cloned KpnI segments demonstrated microheterogeneity among individual members within the same KpnI family. Autoradiograms of capuchin monkey, AGM and human DNA cleaved with HaeIII, AluI or RsaI and hybridized to various cloned human KpnI sequences demonstrated a remarkable conservatism and relative simplicity in the organization of the KpnI families in the genomes of these widely divergent primates. The KpnI 1.2 kb and 1.5 kb families occur in high frequency (15 %) among all plaques in two recombinant human genome libraries. Evidence is presented suggesting that the bulk of the KpnI families occur in the genome as clusters or congeries of higher molecular weight segments ( >2 kb) containing sequences homologous to the low molecular weight segments (1.2 to 1.9 kb).
AB - KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately cloned in the KpnI site of a plasmid pBK5, specially constructed for this purpose. The KpnI clones did not cross-hybridize with cloned, primate alphoid sequences, suggesting that the KpnI families represent sequences separate and distinct from the alphoid DNAs. Secondary restriction analyses of cloned KpnI segments demonstrated microheterogeneity among individual members within the same KpnI family. Autoradiograms of capuchin monkey, AGM and human DNA cleaved with HaeIII, AluI or RsaI and hybridized to various cloned human KpnI sequences demonstrated a remarkable conservatism and relative simplicity in the organization of the KpnI families in the genomes of these widely divergent primates. The KpnI 1.2 kb and 1.5 kb families occur in high frequency (15 %) among all plaques in two recombinant human genome libraries. Evidence is presented suggesting that the bulk of the KpnI families occur in the genome as clusters or congeries of higher molecular weight segments ( >2 kb) containing sequences homologous to the low molecular weight segments (1.2 to 1.9 kb).
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U2 - 10.1093/nar/10.10.3175
DO - 10.1093/nar/10.10.3175
M3 - Article
C2 - 6285290
AN - SCOPUS:0020490627
SN - 0305-1048
VL - 10
SP - 3175
EP - 3193
JO - Nucleic acids research
JF - Nucleic acids research
IS - 10
ER -