Kinetics of hemoglobin S polymerization and gelation under shear

I. Shape of the viscosity progress curve and dependence of delay time and reaction rate on shear rate and temperature

Robin W. Briehl, Peggy Nikolopoulou

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Polymerization and gelation of deoxyhemoglobin S makes red blood cells (RBCs) rigid and is the immediate basis of pathogenesis in sickle cell disease. Hence, characterization of hemoglobin S viscosity and its time-dependent development as RBCs pass through the microvasculature is important in understanding pathogenesis. Because RBCs and the intraerythrocytic milieu in vivo are subject to shear, the shear dependence of polymerization kinetics is also important. In steady-state cone-plate viscometry we find: (1) gelation under shear progresses exponentially with time; (2) shear markedly increases exponential rate and (3) shortens delay time independent of when in the delay time it is applied; (4) shear greatly decreases the temperature dependence of the exponential rate and delay time; (5) simultaneous with its acceleratory effect on polymerization, shear breaks down gel structure. We conclude that shear acts to accelerate gelation by breaking fibers and creating new growing ends, a process that occurs in addition to the homogeneous and heterogeneous nucleation of new fibers that occurs in the absence of shear. Fibers that break are part of a gel network rather than in free solution. The shear dependence of gelation rates means that the critical clinical issue, whether the delay time is long enough and gelation slow enough to permit deoxygenated cells to pass through the microvasculature before they rigidity, depends on in vivo shear rates as well as on degree of unsaturation and hemoglobin concentration.

Original languageEnglish (US)
Pages (from-to)2420-2428
Number of pages9
JournalBlood
Volume81
Issue number9
StatePublished - May 1 1993

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Sickle Hemoglobin
Gelation
Viscosity
Polymerization
Shear deformation
Reaction rates
Time delay
Kinetics
Temperature
Blood
Erythrocytes
Fibers
Microvessels
Gels
Viscosity measurement
Rigidity
Sickle Cell Anemia
Cones
Hemoglobins
Nucleation

ASJC Scopus subject areas

  • Hematology

Cite this

Kinetics of hemoglobin S polymerization and gelation under shear : I. Shape of the viscosity progress curve and dependence of delay time and reaction rate on shear rate and temperature. / Briehl, Robin W.; Nikolopoulou, Peggy.

In: Blood, Vol. 81, No. 9, 01.05.1993, p. 2420-2428.

Research output: Contribution to journalArticle

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