Kinesin-73 is a processive motor that localizes to Rab5-containing organelles

Thomas M. Huckaba, Arne Gennerich, James E. Wilhelm, Athar H. Chishti, Ronald D. Vale

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Drosophila Kinesin-73 (Khc-73), which plays a role in mitotic spindle polarity in neuroblasts, is a metazoan-specific member of the Kinesin-3 family of motors, which includes mammalian KIF1A and Caenorhabditis elegans Unc-104. The mechanism of Kinesin-3 motors has been controversial because some studies have reported that they transport cargo as monomers whereas other studies have suggested a dimer mechanism. Here, we have performed single-molecule motility and cell biological studies of Khc-73. We find that constructs containing the motor and the conserved short stretches of putative coiled-coil-forming regions are predominantly monomeric in vitro, but that dimerization allows for fast, processive movement and high force production (7 piconewtons). In Drosophila cell lines, we present evidence that Khc-73 can dimerize in vivo. We also show that Khc-73 is recruited specifically to Rab5-containing endosomes through its "tail" domain. Our results suggest that the N-terminal half of Khc-73 can undergo a monomer-dimer transition to produce a fast processive motor and that its C-terminal half possesses a specific Rab5-vesicle binding domain.

Original languageEnglish (US)
Pages (from-to)7457-7467
Number of pages11
JournalJournal of Biological Chemistry
Volume286
Issue number9
DOIs
StatePublished - Mar 4 2011

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Kinesin
Organelles
Drosophila
Dimers
Spindle Apparatus
Monomers
Endosomes
Caenorhabditis elegans
Dimerization
Cell Movement
Tail
Cell Line
Cells
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Kinesin-73 is a processive motor that localizes to Rab5-containing organelles. / Huckaba, Thomas M.; Gennerich, Arne; Wilhelm, James E.; Chishti, Athar H.; Vale, Ronald D.

In: Journal of Biological Chemistry, Vol. 286, No. 9, 04.03.2011, p. 7457-7467.

Research output: Contribution to journalArticle

Huckaba, Thomas M. ; Gennerich, Arne ; Wilhelm, James E. ; Chishti, Athar H. ; Vale, Ronald D. / Kinesin-73 is a processive motor that localizes to Rab5-containing organelles. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 9. pp. 7457-7467.
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