Kappa opioid receptor endocytosis by dynorphin peptides

Bryen A. Jordan, Svetlana Cvejic, Lakshmi A. Devi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Internalization and downregulation are important steps in the modulation of receptor function. Recent work with the β2 adrenergic and opioid receptors have implicated these processes in receptor-mediated activation of mitogen-activated protein kinase (MAPK). We have used CHO cells expressing epitope-tagged rat kappa opioid receptors (rKORs) and prodynorphin-derived peptides to characterize the agonist-mediated endocytosis of rKORs and activation of MAPK. Kappa receptor-selective peptides induced receptor internalization and downregulation whereas nonpeptide agonists did not. An examination of the ability of dynorphin A-17-related peptides (lacking C- terminal amino acids) to promote KOR internalization, inhibition of adenylyl cyclase, and MAPK phosphorylation revealed that the N-terminal seven residues play an important role in eliciting these responses. Both dynorphin peptides and nonpeptide agonists induced rapid and robust phosphorylation of MAPKs. Taken together, these results point to a difference in the ability of dynorphin peptides and nonpeptide ligands to promote rKOR endocytosis and support the view that rKOR internalization is not required for MAPK activation.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalDNA and Cell Biology
Volume19
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

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Dynorphins
kappa Opioid Receptor
Endocytosis
Mitogen-Activated Protein Kinases
Peptides
Down-Regulation
Phosphorylation
Peptide Receptors
CHO Cells
Opioid Receptors
Adenylyl Cyclases
Adrenergic Receptors
Epitopes
Ligands
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kappa opioid receptor endocytosis by dynorphin peptides. / Jordan, Bryen A.; Cvejic, Svetlana; Devi, Lakshmi A.

In: DNA and Cell Biology, Vol. 19, No. 1, 2000, p. 19-27.

Research output: Contribution to journalArticle

Jordan, Bryen A. ; Cvejic, Svetlana ; Devi, Lakshmi A. / Kappa opioid receptor endocytosis by dynorphin peptides. In: DNA and Cell Biology. 2000 ; Vol. 19, No. 1. pp. 19-27.
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