Isopentenyl-diphosphate isomerase is essential for viability of Caenorhabditis elegans

John Yochem, David H. Hall, Leslie R. Bell, Edward M. Hedgecock, Robert K. Herman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Homozygosity for a mutation in the idi-1 gene of Caenorhabditis elegans results in paralysis during the first larval stage, followed by an arrest of growth and development late in the first larval stage. Apoptotic corpses, which are apparently the result of normal programmed cell death, persist in the arrested larvae. In genetic mosaics, an additional defect becomes evident upon examination with Nomarski optics: cells that are genotypically mutant enlarge, and their cytoplasm becomes dimpled. Electron microscopy indicates that the dimpling reflects an accumulation of many enlarged lysosomes and autophagosomes. The mosaics demonstrate that the lethal mutation acts cell autonomously with respect to this vesicular abnormality and that there is a maternal effect with respect to the time of developmental arrest of mutant progeny. Cloning of the gene reveals that it is the only gene in C. elegans for isopentenyl-diphosphate isomerase, an enzyme that is important for the synthesis of lipophilic molecules, including farnesyl and geranyl diphosphates.

Original languageEnglish (US)
Pages (from-to)158-166
Number of pages9
JournalMolecular Genetics and Genomics
Volume273
Issue number2
DOIs
StatePublished - Apr 2005

Fingerprint

Isomerases
Caenorhabditis elegans
Genes
Mutation
Lysosomes
Growth and Development
Cadaver
Paralysis
Larva
Organism Cloning
Electron Microscopy
Cytoplasm
Cell Death
Enzymes
isopentenyl pyrophosphate

Keywords

  • Autophagy
  • idi-1
  • Lysosomal storage disease
  • Prenylation

ASJC Scopus subject areas

  • Genetics

Cite this

Isopentenyl-diphosphate isomerase is essential for viability of Caenorhabditis elegans. / Yochem, John; Hall, David H.; Bell, Leslie R.; Hedgecock, Edward M.; Herman, Robert K.

In: Molecular Genetics and Genomics, Vol. 273, No. 2, 04.2005, p. 158-166.

Research output: Contribution to journalArticle

Yochem, John ; Hall, David H. ; Bell, Leslie R. ; Hedgecock, Edward M. ; Herman, Robert K. / Isopentenyl-diphosphate isomerase is essential for viability of Caenorhabditis elegans. In: Molecular Genetics and Genomics. 2005 ; Vol. 273, No. 2. pp. 158-166.
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