Isolation of neonatal rat cortical astrocytes for primary cultures.

J. W. Allen; L. A. Mutkus; M. Aschner

Isolation of neonatal rat cortical astrocytes for primary cultures.

J. W. Allen, L. A. Mutkus, M. Aschner

Research output: Contribution to journal › Article › peer-review

Abstract

There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.

Original language	English (US)
Pages (from-to)	Unit12.4
Journal	Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
Volume	Chapter 12
State	Published - 2001
Externally published	Yes

ASJC Scopus subject areas

Toxicology

Cite this

@article{eddc2b03387a4bb8906cf6c5864be8d8,

title = "Isolation of neonatal rat cortical astrocytes for primary cultures.",

abstract = "There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.",

author = "Allen, {J. W.} and Mutkus, {L. A.} and M. Aschner",

year = "2001",

language = "English (US)",

volume = "Chapter 12",

pages = "Unit12.4",

journal = "Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]",

issn = "1934-9254",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Isolation of neonatal rat cortical astrocytes for primary cultures.

AU - Allen, J. W.

AU - Mutkus, L. A.

AU - Aschner, M.

PY - 2001

Y1 - 2001

N2 - There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.

AB - There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.

UR - http://www.scopus.com/inward/record.url?scp=84874217242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874217242&partnerID=8YFLogxK

M3 - Article

C2 - 23045033

AN - SCOPUS:84874217242

SN - 1934-9254

VL - Chapter 12

SP - Unit12.4

JO - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]

JF - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]

ER -

Isolation of neonatal rat cortical astrocytes for primary cultures.

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this