Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer

Liming Yuan, Jidong Shan, Dwight De Risi, John Broome, John Lovecchio, David Gal, Vincent Vinciguerra, Xu Hao-Peng

Research output: Contribution to journalArticle

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Abstract

A novel gene, testes-specific protease 50 (TSP50), was isolated from a human testes cDNA library by using a genomic DNA probe, BR50. BR50 was isolated by a modified representational difference analysis (RDA) technique due to its hypomethylated feature in a breast cancer biopsy. This altered DNA methylation status was also detected by BR50 in other breast and some ovarian cancer tissues. The TSP50 gene product is a homologue to several human proteases, which indicates that it may encode a protease-like protein. Northern analysis of 16 different types of normal human tissues suggests that TSP50 was highly and specifically expressed in human testes, which indicates that it might possess a unique biological function(s) in that organ. Methylation status analysis in normal human testes and other tissues showed a correlation between DNA methylation and gene expression. Most importantly, reverse transcription-PCR analysis of 18 paired breast cancer tissues found that in 28% of the cancer samples, the TSP50 gene was differentially expressed. The possibility that TSP50 may be an oncogene is presently under investigation.

Original languageEnglish (US)
Pages (from-to)3215-3221
Number of pages7
JournalCancer Research
Volume59
Issue number13
StatePublished - Jul 1 1999
Externally publishedYes

Fingerprint

Breast Neoplasms
DNA
Testis
Genes
DNA Methylation
Peptide Hydrolases
DNA Probes
Testicular Neoplasms
Gene Library
Oncogenes
Ovarian Neoplasms
Methylation
Reverse Transcription
Breast
testis-specific protease 50
Biopsy
Gene Expression
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yuan, L., Shan, J., De Risi, D., Broome, J., Lovecchio, J., Gal, D., ... Hao-Peng, X. (1999). Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer. Cancer Research, 59(13), 3215-3221.

Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer. / Yuan, Liming; Shan, Jidong; De Risi, Dwight; Broome, John; Lovecchio, John; Gal, David; Vinciguerra, Vincent; Hao-Peng, Xu.

In: Cancer Research, Vol. 59, No. 13, 01.07.1999, p. 3215-3221.

Research output: Contribution to journalArticle

Yuan, L, Shan, J, De Risi, D, Broome, J, Lovecchio, J, Gal, D, Vinciguerra, V & Hao-Peng, X 1999, 'Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer', Cancer Research, vol. 59, no. 13, pp. 3215-3221.
Yuan L, Shan J, De Risi D, Broome J, Lovecchio J, Gal D et al. Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer. Cancer Research. 1999 Jul 1;59(13):3215-3221.
Yuan, Liming ; Shan, Jidong ; De Risi, Dwight ; Broome, John ; Lovecchio, John ; Gal, David ; Vinciguerra, Vincent ; Hao-Peng, Xu. / Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer. In: Cancer Research. 1999 ; Vol. 59, No. 13. pp. 3215-3221.
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