Isolation and expression of scabrous, a gene regulating neurogenesis in Drosophila

Marek Mlodzik, Nicholas E. Baker, Gerald M. Rubin

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Mutations in the Drosophila scabrous (sea) gene affect eye and bristle development, leading to irregular spacing of ommatidia and bristle duplications in the adult fly. We have cloned the sea gene by P-element tagging. The sea transcription unit is 12 kb and consists of four exons that are joined in a 3.2-kb mRNA. In an enhancer trap screen we have isolated several P[lacZ] insertions close to the sea transcription start site. We have examined the expression pattern of sea by in situ hybridization to sea transcripts, by β-galactosidase localization in the P[lacZ] lines, and by immunocytochemistry with an anti-sca antiserum. During embryogenesis, sea is expressed in a dynamic pattern associated with neural development. During imaginal development, sea is mainly expressed in the R8 photoreceptor precursor cells in the eye imaginal disc and in sensory organ precursor cells in other discs. In the wing disc, sea expression is coextensive with the anlagen for bristles and is controlled by genes of the achaete-scute complex. Based on its loss-of-function phenotype, expression pattern, and the predicted structure of its product, a secreted peptide with homology to the fibrinogen gene family, we propose that sea encodes a signal involved in lateral inhibition within individual domains of the developing nervous system.

Original languageEnglish (US)
Pages (from-to)1848-1861
Number of pages14
JournalGenes and Development
Volume4
Issue number11
StatePublished - 1990
Externally publishedYes

Fingerprint

Neurogenesis
Oceans and Seas
Drosophila
Genes
Achaete-Scute Complex Genome Region
Galactosidases
Imaginal Discs
Photoreceptor Cells
Transcription Initiation Site
Diptera
Fibrinogen
Nervous System
Embryonic Development
In Situ Hybridization
Immune Sera
Exons
Immunohistochemistry
Phenotype
Messenger RNA
Peptides

Keywords

  • Drosophila
  • Enhancer trap lines
  • Genetic interactions
  • Neurogenesis
  • Pattern formation
  • Scabrous

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Isolation and expression of scabrous, a gene regulating neurogenesis in Drosophila. / Mlodzik, Marek; Baker, Nicholas E.; Rubin, Gerald M.

In: Genes and Development, Vol. 4, No. 11, 1990, p. 1848-1861.

Research output: Contribution to journalArticle

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