Isolation and characterization of the human gene encoding Ito: Further diversity by alternative mRNA splicing

Wei Kong, Sunny Po, Toshio Yamagishi, M. Dominique Ashen, Gail Stetten, Gordon F. Tomaselli

Research output: Contribution to journalArticle

Abstract

The transient outward K+ current (Ito) in the heart is responsible for the initial phase of repolarization and for setting the plateau voltage of the ventricular action potential. Recently, Kv4.3 has emerged as the leading candidate α-subunit gene that underlies Ito in larger mammals such as dogs and humans. We have cloned the human Kv4.3 homolog and describe a carboxyl-terminal splice variant that inserts 19 amino acids with a consensus protein kinase C (PKC) phosphorylation site into the protein after the last membrane-spanning segment. The coding region of Kv4.3 is comprised of at least five exons and is located on chromosome 1p13.3. In the basal state the basic biophysical properties of both of the splice variants are identical.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume44
Issue number6
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • Chromosome 1
  • Fluorescence in situ hybridization
  • Heterologous expression
  • Kv4.2
  • Kv4.3
  • Potassium channel
  • Xenopus oocytes

ASJC Scopus subject areas

  • Physiology

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